Enzymatic manipulation of DNA - Nanomaterial constructs

被引：61

作者：

Yun, CS ^{[1
]}

Khitrov, GA ^{[1
]}

Vergona, DE ^{[1
]}

Reich, NO ^{[1
]}

Strouse, GF ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 26期

关键词：

D O I：

10.1021/ja025971o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The demonstration and control of biofunction between inorganic nanomaterials and biological scaffolding is crucial to the development of the field of biomaterials. Although unique hierarchical structures can be generated, the impact of nanosized materials on the biological activity of DNA-protein interactions is relatively unknow. Using highly selective proteins that induce sequence-specific conformational perturbations within DNA, we demonstrate the absolute maintenance of biofunction for biomaterials composed of duplex DNA appended with 1.4-nm Au particles. Enzyme activity and DNA binding affinities (Kd) are unaltered by the nanoparticle-DNA conjugates. Our results provide a foundation for interfacing more complex and diverse protein-DNA-systems. Copyright © 2002 American Chemical Society.

引用

页码：7644 / 7645

页数：2

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