Enhancing effect of ultraviolet A on ornithine decarboxylase induction and dermatitis evoked by 12-o-tetradecanoylphorbol-13-acetate and its inhibition by curcumin in mouse skin

被引：29

作者：

Ishizaki, C ^{[1
]}

Oguro, T ^{[1
]}

Yoshida, T ^{[1
]}

Wen, CQ ^{[1
]}

Sueki, H ^{[1
]}

Iijima, M ^{[1
]}

机构：

[1] SHOWA UNIV, SCH PHARMACEUT SCI, DEPT BIOCHEM TOXICOL, TOKYO, JAPAN

来源：

DERMATOLOGY | 1996年 / 193卷 / 04期

关键词：

ultraviolet A; ornithine decarboxylase; 12-o-tetradecanoylphorbol-13-acetate; S-adenosylmethionine decarboxylase; curcumin; tumor promoter;

D O I：

10.1159/000246276

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background: Previous studies have demonstrated appreciable tumor induction in mouse skin by daily irradiation with high-power long-wavelength ultraviolet A (UVA). Object: The aim of the present study was to examine the enhancing effects of UVA on changes in mouse skin mediated by the tumor promoter 12-o-tetradecanoylphorbol- 13-acetate (TPA) by measurement of ornithine decarboxylase (ODC) activity and morphometric analysis. In addition, we examined the inhibitory effects of curcumin, a component of turmeric, on these changes. Method: ODC activity in the epidermis of CD-1 mice was determined by the method of Russell and Snyder. Epidermal and dermal thickness, and the number of dermal infiltrating inflammatory cells were quantified using a computer-assisted image analyzer. Results: A combination of topical TPA application and UVA irradiation produced a greater increment of ODC activity at 4 h than TPA alone (p < 0.05). Histopathologically, TPA plus UVA tended to increase the dermal infiltrating inflammatory cells in contrast to TPA alone. Pretreatment of mice with curcumin significantly abrogated the TPA-induced changes in ODC activity and the dermal infiltrating inflammatory cells as well as the TPA plus UVA-mediated enhancement of these changes. Conclusion: Our data indicate that UVA irradiation (18.72 J/cm(2)) significantly enhances ODC induction at an early stage (4-6 h) after topical application of TPA, and aggravates the dermatitis elicited by TPA. Pretreatment with curcumin significantly inhibits these enhancing effects.

引用

页码：311 / 317

页数：7

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