Transmembrane segments of syntaxin line the fusion pore of Ca2+-triggered exocytosis

被引：254

作者：

Han, X ^{[1
]}

Wang, CT ^{[1
]}

Bai, JH ^{[1
]}

Chapman, ER ^{[1
]}

Jackson, MB ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Physiol, Madison, WI 53706 USA

来源：

SCIENCE | 2004年 / 304卷 / 5668期

关键词：

D O I：

10.1126/science.1095801

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The fusion pore of regulated exocytosis is a channel that connects and spans the vesicle and plasma membranes. The molecular composition of this important intermediate structure of exocytosis is unknown. Here, we found that mutations of some residues within the transmembrane segment of syntaxin (Syx), a plasma membrane protein essential for exocytosis, altered neurotransmitter flux through fusion pores and altered pore conductance. The residues that influenced fusion-pore flux lay along one face of an alpha-helical model. Thus, the fusion pore is formed at least in part by a circular arrangement of 5 to 8 Syx transmembrane segments in the plasma membrane.

引用

页码：289 / 292

页数：4

共 30 条

[1] cGMP and S-nitrosylation: two routes for modulation of neuronal excitability by NO [J].