Characterization of microsatellite loci for five members of the minnow family Cyprinidae found in the Sacramento-San Joaquin Delta and its tributaries

被引：44

作者：

Baerwald, MR ^{[1
]}

May, B ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 03期

关键词：

Cyprinidae; microsatellites; primers; Sacramento-San Joaquin Delta;

D O I：

10.1111/j.1471-8286.2004.00661.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two microsatellite-enriched libraries [(CAGA)(n), (TAGA)(n)] were constructed using pooled DNA from three cyprinid species native to the Sacramento-San Joaquin Delta of California: Sacramento splittail (Pogonichthys macrolepidotus); Sacramento pikeminnow (Ptychocheilus grandis); and tui chub (Siphateles bicolor). Primers were designed for 105 loci and tested for levels of polymorphism in five cyprinid species found in the Delta: Sacramento splittail, Sacramento pikeminnow, tui chub, hitch (Lavinia exilicauda), and Sacramento blackfish (Orthodon microlepidotus). Fifty-one loci were polymorphic for at least one species and 31 loci were polymorphic for multiple species. The number of polymorphic loci per species ranged from 16 to 26.

引用

页码：385 / 390

页数：6

共 6 条

[1]

Goudet J., 2002, FSTAT, a Program to Estimate and Test Gene Diversities and Fixation Indices

[2] Statistical confidence for likelihood-based paternity inference in natural populations [J].