Tissue-specific and imprinted epigenetic modifications of the human NDN gene

被引：45

作者：

Lau, JCY ^{[1
]}

Hanel, ML ^{[1
]}

Wevrick, R ^{[1
]}

机构：

[1] Univ Alberta, Dept Med Genet, Edmonton, AB T6G 2H7, Canada

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 11期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

D O I：

10.1093/nar/gkh671

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Allele-specific DNA methylation, histone acetylation and histone methylation are recognized as epigenetic characteristics of imprinted genes and imprinting centers (ICs). These epigenetic modifications are also used to regulate tissue-specific gene expression. Epigenetic differences between alleles can be significant either in the function of the IC or in the cis-acting effect of the IC on 'target' genes responding to it. We have now examined the epigenetic characteristics of NDN, a target gene of the chromosome 15q11-q13 Prader-Willi Syndrome IC, using sodium bisulfite sequencing to analyze DNA methylation and chromatin immunoprecipitation to analyze histone modifications. We observed a bias towards maternal allele-specific DNA hypermethylation of the promoter CpG island of NDN, independent of tissue-specific transcriptional activity. We also found that NDN lies in a domain of paternal allele-specific histone hyperacetylation that correlates with transcriptional state, and a domain of differential histone H3 lysine 4 di- and tri-methylation that persists independent of transcription. These results suggest that DNA methylation and histone H3 lysine 4 methylation are persistent markers of imprinted gene regulation while histone acetylation participates in tissue-specific activity and silencing in somatic cells.

引用

页码：3376 / 3382

页数：7

共 53 条

[1]

Ausubel F. M., 1993, CURRENT PROTOCOLS MO, V1

[2] Clonal maintenance of imprinted expression of SNRPN and IPW in normal lymphocytes:: correlation with allele-specific methylation of SNRPN intron 1 but not intron 7 [J].

Balmer, D ;

LaSalle, JM .

HUMAN GENETICS, 2001, 108 (02) :116-122

[3] LOSS OF METHYLATION ACTIVATES XIST IN SOMATIC BUT NOT IN EMBRYONIC-CELLS [J].

BEARD, C ;

LI, E ;

JAENISCH, R .

GENES & DEVELOPMENT, 1995, 9 (19) :2325-2334

[4] Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes [J].

Boggs, BA ;

Cheung, P ;

Heard, E ;

Spector, DL ;

Chinault, AC ;

Allis, CD .

NATURE GENETICS, 2002, 30 (01) :73-76

[5] Mechanisms of genomic imprinting [J].

Brannan, CI ;

Bartolomei, MS .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (02) :164-170

[6] Methylation matters: a new spin on maspin [J].

Costello, JF ;

Vertino, PM .

NATURE GENETICS, 2002, 31 (02) :123-124

[7] Imprint switching on human chromosome 15 may involve alternative transcripts of the SNRPN gene [J].

Dittrich, B ;

Buiting, K ;

Korn, B ;

Rickard, S ;

Buxton, J ;

Saitoh, S ;

Nicholls, RD ;

Poustka, A ;

Winterpacht, A ;

Zabel, B ;

Horsthemke, B .

NATURE GENETICS, 1996, 14 (02) :163-170

[8]

El-Osta A, 2000, GENE EXPRESSION, V9, P63

[9]

ELMAARRI O, 2001, NAT GENET, V27, P241

[10] Imprinting and the epigenetic asymmetry between parental genomes [J].

Ferguson-Smith, AC ;

Surani, MA .

SCIENCE, 2001, 293 (5532) :1086-1089

← 1 2 3 4 5 6 →