A novel and simple alcohol-free sol-gel route for encapsulation of labile proteins

被引：128

作者：

Ferrer, ML

del Monte, F

Levy, D

机构：

[1] CSIC, ICMM, E-28049 Madrid, Spain

[2] INTA, LINES, Madrid 28850, Spain

来源：

CHEMISTRY OF MATERIALS | 2002年 / 14卷 / 09期

关键词：

D O I：

10.1021/cm025562r

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This work presents a novel and simple aqueous sol-gel route for the encapsulation of biomolecules, which are labile to the alcohol that results as a byproduct of the matrix formation. The controlled vacuum evaporation of the alcohol by rotavapor methods provides an aqueous sol in which the activity of labile biomolecules is preserved. The versatility of this approach is remarkable since it allows the preparation of pure silica matrixes as well as ormosils.

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页码：3619 / +

页数：4

相关论文

共 26 条

[1] LIQUID-CRYSTALLINE PHASES AS TEMPLATES FOR THE SYNTHESIS OF MESOPOROUS SILICA [J].

ATTARD, GS ;

GLYDE, JC ;

GOLTNER, CG .

NATURE, 1995, 378 (6555) :366-368

[2] ENZYMES AND OTHER PROTEINS ENTRAPPED IN SOL-GEL MATERIALS [J].

AVNIR, D ;

BRAUN, S ;

LEV, O ;

OTTOLENGHI, M .

CHEMISTRY OF MATERIALS, 1994, 6 (10) :1605-1614

[3] Effects of encapsulation in sol-gel silica glass on esterase activity, conformational stability, and unfolding of bovine carbonic anhydrase II [J].

Badjic, JD ;

Kostic, NM .

CHEMISTRY OF MATERIALS, 1999, 11 (12) :3671-3679

[4] Aqueous sol-gel process for protein encapsulation [J].

Bhatia, RB ;

Brinker, CJ ;

Gupta, AK ;

Singh, AK .

CHEMISTRY OF MATERIALS, 2000, 12 (08) :2434-2441

[5] Structural and conformational stability of horseradish peroxidase: Effect of temperature and pH [J].

Chattopadhyay, K ;

Mazumdar, S .

BIOCHEMISTRY, 2000, 39 (01) :263-270

[6] Reagentless amperometric determination of hydrogen peroxide by silica sol-gel modified biosensor [J].

Chut, SL ;

Li, J ;

Tan, SN .

ANALYST, 1997, 122 (11) :1431-1434

[7] Sol-gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes [J].

Coiffier, A ;

Coradin, T ;

Roux, C ;

Bouvet, OMM ;

Livage, J .

JOURNAL OF MATERIALS CHEMISTRY, 2001, 11 (08) :2039-2044

[8] Ordered mesoporous silicas prepared from both micellar solutions and liquid crystal phases [J].

Coleman, NRB ;

Attard, GS .

MICROPOROUS AND MESOPOROUS MATERIALS, 2001, 44 :73-80

[9] Preservation of the native structure in myoglobin at low pH by sol-gel encapsulation [J].

Das, TK ;

Khan, I ;

Rousseau, DL ;

Friedman, JM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (39) :10268-10269

[10] ENCAPSULATION OF PROTEINS IN TRANSPARENT POROUS SILICATE-GLASSES PREPARED BY THE SOL-GEL METHOD [J].

ELLERBY, LM ;

NISHIDA, CR ;

NISHIDA, F ;

YAMANAKA, SA ;

DUNN, B ;

VALENTINE, JS ;

ZINK, JI .

SCIENCE, 1992, 255 (5048) :1113-1115