Nano-patterned SERS substrate: Application for protein analysis vs. temperature

被引：211

作者：

Das, Gobind ^{[1
,2
]}

Mecarini, Federico ^{[1
,2
]}

Gentile, Francesco ^{[1
,2
]}

De Angelis, Francesco ^{[1
,2
]}

Kumar, Mohan H. G. ^{[3
]}

Candeloro, Patrizio ^{[1
]}

Liberale, Carlo ^{[1
,2
]}

Cuda, Giovanni ^{[2
,3
]}

Di Fabrizio, Enzo ^{[1
,2
,4
]}

机构：

[1] Magna Graecia Univ Catanzaro, Dipartimento Med Sperimentale & Clin, Lab BIONEM, Catanzaro, Italy

[2] CalMED Srl, Sede Catanzaro Cda Mula Loc Germaneto, I-88100 Catanzaro, Italy

[3] Magna Graecia Univ Catanzaro, Dipartimento Med Sperimentale & Clin, Prote Lab, Catanzaro, Italy

[4] INFM TASC SS, Basovizza Trieste, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 06期

关键词：

SERS; Proteins; Difference and 2D-correlation Raman spectroscopy; Temperature variation; RESONANCE RAMAN-SPECTROSCOPY; VIRUS STRUCTURE; CONFORMATION; DIFFERENCE; SCATTERING; STABILITY; LYSOZYME; SPECTRA; ACID;

D O I：

10.1016/j.bios.2008.08.050

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have illustrated the fabrication of nano-structures as a surface enhanced Raman scattering (SERS) substrate using electro-plating and electron-beam lithography techniques to obtain an array of gold nanograin-aggregate structures of diameter ranging between 80 and 100nm with interstitial gap of 10-30 nm. The nanostructure based SERS substrate permits us to have better control and reproducibility on generation of plasmon polaritons. The calculation shows the possible detection of myoglobin concentration down to attomole. This SERS substrate is used to investigate the structural changes of different proteins; lysozyme, ribonuclease-B, bovin serum albumin and myoglobin in the temperature range between -65 and 90 degrees C. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1693 / 1699

页数：7

共 36 条

[1] THE STRUCTURES OF THE MONOCLINIC AND ORTHORHOMBIC FORMS OF HEN EGG-WHITE LYSOZYME AT 6-A RESOLUTION [J].