BACE1 interacts with nicastrin

被引：26

作者：

Hattori, C

Asai, M

Oma, Y

Kino, Y

Sasagawa, N

Saido, TC

Maruyama, K

Ishiura, S ^{[1
]}

机构：

[1] Univ Tokyo, Dept Life Sci, Grad Sch Arts & Sci, Meguro Ku, Tokyo 1538902, Japan

[2] RIKEN, Brain Sci Inst, Wako, Saitama 3510198, Japan

[3] Saitama Med Sch, Dept Pharmacol, Moroyama, Saitama 3500495, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 04期

关键词：

Alzheimer's disease; APP; A beta; beta-secretase; gamma-secretase; BACE1; nicastrin;

D O I：

10.1016/S0006-291X(02)00351-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-Amyloid peptide (Abeta) is generated through the proteolytic cleavage of beta-amyloid precursor protein (APP) by beta- and gamma-secretases. The beta-secretase, BACE1, initiates Abeta formation followed by gamma-cleavage within the APP transmembrane domain. Although BACE1 localizes in the transGolgi network (TGN), its physiological substrates and modulators are not known, In addition, the relationship to other secretase(s) also remains unidentified. Here, we demonstrate that BACE1 binds to nicastrin, a component of gamma-secretase complexes, in vitro, and that nicastrin activates beta-secretase activity in COS-7 cells. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

收藏

页码：1228 / 1232

页数：5

相关论文

共 29 条

[1]

[Anonymous], 1907, Centralblatt fur Nervenheilkunde Psychiatrie

[2] Expression analysis of BACE2 in brain and peripheral tissues [J].

Bennett, BD ;

Babu-Khan, S ;

Loeloff, R ;

Louis, JC ;

Curran, E ;

Citron, M ;

Vassar, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (27) :20647-20651

[3] A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's β-secretase [J].

Bennett, BD ;

Denis, P ;

Haniu, M ;

Teplow, DB ;

Kahn, S ;

Louis, JC ;

Citron, M ;

Vassar, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (48) :37712-37717

[4] Evidence that tumor necrosis factor α converting enzyme is involved in regulated α-secretase cleavage of the Alzheimer amyloid protein precursor [J].

Buxbaum, JD ;

Liu, KN ;

Luo, YX ;

Slack, JL ;

Stocking, KL ;

Peschon, JJ ;

Johnson, RS ;

Castner, BJ ;

Cerretti, DP ;

Black, RA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (43) :27765-27767

[5] BACE1 is the major β-secretase for generation of Aβ peptides by neurons [J].

Cai, HB ;

Wang, YS ;

McCarthy, D ;

Wen, HJ ;

Borchelt, DR ;

Price, DL ;

Wong, PC .

NATURE NEUROSCIENCE, 2001, 4 (03) :233-234

[6] Presenilin-1 differentially facilitates endoproteolysis of the β-amyloid precursor protein and Notch [J].

Capell, A ;

Steiner, H ;

Romig, H ;

Keck, S ;

Baader, M ;

Grim, MG ;

Baumeister, R ;

Haass, C .

NATURE CELL BIOLOGY, 2000, 2 (04) :205-211

[7] Deficiency of presenilin-1 inhibits the normal cleavage of amyloid precursor protein [J].

De Strooper, B ;

Saftig, P ;

Craessaerts, K ;

Vanderstichele, H ;

Guhde, G ;

Annaert, W ;

Von Figura, K ;

Van Leuven, F .

NATURE, 1998, 391 (6665) :387-390

[8] BACE2, a β-secretase homolog, cleaves at the β site and within the amyloid-β region of the amyloid-β precursor protein [J].

Farzan, M ;

Schnitzler, CE ;

Vasilieva, N ;

Leung, D ;

Choe, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) :9712-9717

[9] ALZHEIMERS-DISEASE - INITIAL REPORT OF THE PURIFICATION AND CHARACTERIZATION OF A NOVEL CEREBROVASCULAR AMYLOID PROTEIN [J].

GLENNER, GG ;

WONG, CW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 120 (03) :885-890

[10] CELLULAR PROCESSING OF BETA-AMYLOID PRECURSOR PROTEIN AND THE GENESIS OF AMYLOID BETA-PEPTIDE [J].

HAASS, C ;

SELKOE, DJ .

CELL, 1993, 75 (06) :1039-1042