Selective photocleavage of DNA and RNA by anthraquinone derivatives: Targeting the single-strand region of hairpin structures

被引：13

作者：

Fernandez-Saiz, M

Henderson, PT

Wilson, WD

Schuster, GB ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[2] Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1999年 / 70卷 / 06期

关键词：

D O I：

10.1111/j.1751-1097.1999.tb08292.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A tetracationic anthraquinone derivative (27AQS2) binds to hairpin DNA and RNA. Ultraviolet irradiation of the bound quinone causes cleavage in the loop region of both oligonucleotides and at guanines in the stem region of the DNA hairpin. The absence of observable strand cleavage at guanines in the RNA hairpin suggests that either aniline treatment does not cause cleavage at damaged guanines in RNA or that radical cation migration does not occur readily in RNA duplexes. The ability to target the single-stranded regions of DNA and RNA structures is an important property of this photonuclease.

引用

收藏

页码：847 / 852

页数：6

相关论文

共 26 条

[1] Long-range oxidation of guanine by Ru(III) in duplex DNA [J].

Arkin, MR ;

Stemp, EDA ;

Pulver, SC ;

Barton, JK .

CHEMISTRY & BIOLOGY, 1997, 4 (05) :389-400

[2] Photocleavage of nucleic acids [J].

Armitage, B .

CHEMICAL REVIEWS, 1998, 98 (03) :1171-1200

[3] Anthraquinone photonucleases: A surprising role for chloride in the sequence-neutral cleavage of DNA and the footprinting of minor groove-bound ligands [J].

Armitage, B ;

Schuster, GB .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (02) :164-170

[4] CATIONIC ANTHRAQUINONE DERIVATIVES AS CATALYTIC DNA PHOTONUCLEASES - MECHANISMS FOR DNA-DAMAGE AND QUINONE RECYCLING [J].

ARMITAGE, B ;

YU, CJ ;

DEVADOSS, C ;

SCHUSTER, GB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (22) :9847-9859

[5] Anthraquinone photonuclease structure determines its mode of binding to DNA and the cleavage chemistry observed [J].

Breslin, DT ;

Coury, JE ;

Anderson, JR ;

McFailIsom, L ;

Kan, YZ ;

Williams, LD ;

Bottomley, LA ;

Schuster, GB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (21) :5043-5044

[6] Structural modification changes the DNA binding mode of cation-substituted anthraquinone photonucleases: Association by intercalation or minor groove binding determines the DNA cleavage efficiency [J].

Breslin, DT ;

Yu, CJ ;

Ly, D ;

Schuster, GB .

BIOCHEMISTRY, 1997, 36 (34) :10463-10473

[7] COMPARATIVE-STUDY OF OXIDATION OF NUCLEIC-ACID COMPONENTS BY HYDROXYL RADICALS, SINGLET OXYGEN AND SUPEROXIDE ANION RADICALS [J].

CADET, J ;

TEOULE, R .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1978, 28 (4-5) :661-667

[8] Effects of secondary structure on DNA and RNA cleavage by diplatinum(II) [J].

Carter, PJ ;

Breiner, KM ;

Thorp, HH .

BIOCHEMISTRY, 1998, 37 (39) :13736-13743

[9] FORMATION OF 8-HYDROXY(DEOXY)GUANOSINE AND GENERATION OF STRAND BREAKS AT GUANINE RESIDUES IN DNA BY SINGLET OXYGEN [J].

DEVASAGAYAM, TPA ;

STEENKEN, S ;

OBENDORF, MSW ;

SCHULZ, WA ;

SIES, H .

BIOCHEMISTRY, 1991, 30 (25) :6283-6289

[10] Intramolecular photoinduced electron transfer to anthraquinones linked to duplex DNA: The effect of gaps and traps on long-range radical cation migration [J].

Gasper, SM ;

Schuster, GB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (52) :12762-12771