Mitochondria do not contain lipid rafts, and lipid rafts do not contain mitochondrial proteins

被引:57
作者
Zheng, Yu Zi
Berg, Kyra B.
Foster, Leonard J. [1 ]
机构
[1] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V6T 1Z4, Canada
关键词
detergent-resistant membranes; membrane proteins; stable isotope labeling by amino acids in cell culture; quantitative proteomics; mass spectrometry; methyl-beta-cyclodextrin; protein correlation profiling; TANDEM MASS-SPECTROMETRY; QUANTITATIVE PROTEOMIC ANALYSIS; DETERGENT-RESISTANT MEMBRANES; GPI-ANCHORED PROTEINS; AFFINITY TAG ICAT; PLASMA-MEMBRANE; CELL-MEMBRANES; LIQUID-CHROMATOGRAPHY; SOFTWARE TOOLS; MICRODOMAINS;
D O I
10.1194/jlr.M800658-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipid rafts are membrane microdomains involved in many cellular functions, including transduction of cellular signals and cell entry by pathogens. Lipid rafts can be enriched biochemically by extraction in a nonionic detergent at low temperature, followed by floatation on a sucrose density gradient. Previous proteomic studies of such detergent-resistant membranes (DRMs) are in disagreement about the presence of mitochondrial proteins in raft components. jlr Here, we approach the status of mitochondrial proteins in DRM preparations by employing stable isotope labeling by amino acids in cell culture to evaluate the composition of differentially purified subcellular fractions as well as high-resolution linear density gradients. Our data demonstrate that F-1/F-0 ATPase subunits, voltage-dependent anion selective channels, and other mitochondrial proteins are at best partially copurifying contaminants of raft preparations.-Zheng, Y. Z., K. B. Berg, and L. J. Foster. Mitochondria do not contain lipid rafts, and lipid rafts do not contain mitochondrial proteins. J. Lipid Res. 2009. 50: 988-998.
引用
收藏
页码:988 / 998
页数:11
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