Phylogenetic analysis of the small hydrophobic (SH) gene of mumps virus in Korea: Identification of a new genotype

被引:28
作者
Kim, SH
Song, KJ
Shin, YK
Kim, JH
Choi, SM
Park, KS
Baek, LJ
Lee, YJ
Song, JW
机构
[1] Korea Univ, Coll Med, Dept Microbiol, Sungbuk Ku, Seoul 136705, South Korea
[2] Korea Univ, Coll Med, Inst Viral Dis, Sungbuk Ku, Seoul 136705, South Korea
[3] Korea Univ, Coll Med, Dept Pediat, Sungbuk Ku, Seoul 136705, South Korea
[4] Korea Natl Inst Hlth, Eunpyung Ku, Seoul 122701, South Korea
关键词
mumps virus; RT-PCR; SH gene;
D O I
10.1111/j.1348-0421.2000.tb02479.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Viral RNAs extracted from fifteen mumps virus isolated from throat swab, saliva, blood, urine or CSF during mumps epidemics between 1997-1998 in Korea were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and compared by nucleotide sequencing of the small hydrophobic (SH) gene. The deduced amino acid sequences of the SH gene were aligned with the published sequences of mumps virus isolated in different geographic areas, A comparison of the SH gene of mumps viruses in Korea indicated 96.2-100% and 91.2-100% similarity at the nucleotide and amino acid levels, respectively. Phylogenetic analysis, using the neighbor-joining method, showed that Korean mumps virus strains formed a genetically distinct monophyletic group from previously reported genotypes based on the 315-bp length nucleotide and 57 deduced amino acid sequences of the SH gene, and possibly be designated as a new genotype (I).
引用
收藏
页码:173 / 177
页数:5
相关论文
共 14 条
[1]   Clustering of mumps virus isolates by SH gene sequence only partially reflects geographical origin [J].
Afzal, MA ;
Buchanan, J ;
Heath, AB ;
Minor, PD .
ARCHIVES OF VIROLOGY, 1997, 142 (02) :227-238
[2]   MOLECULAR-CLONING AND CHARACTERIZATION OF 6 GENES, DETERMINATION OF GENE ORDER AND INTERGENIC SEQUENCES AND LEADER SEQUENCE OF MUMPS-VIRUS [J].
ELANGO, N ;
VARSANYI, TM ;
KOVAMEES, J ;
NORRBY, E .
JOURNAL OF GENERAL VIROLOGY, 1988, 69 :2893-2900
[3]   MESSENGER-RNA SEQUENCE AND DEDUCED AMINO-ACID SEQUENCE OF THE MUMPS-VIRUS SMALL HYDROPHOBIC PROTEIN GENE [J].
ELANGO, N ;
KOVAMEES, J ;
VARSANYI, TM ;
NORRBY, E .
JOURNAL OF VIROLOGY, 1989, 63 (03) :1413-1415
[4]   NUCLEOTIDE-SEQUENCE OF THE MATRIX, FUSION AND PUTATIVE SH PROTEIN GENES OF MUMPS-VIRUS AND THEIR DEDUCED AMINO-ACID SEQUENCES [J].
ELLIOTT, GD ;
AFZAL, MA ;
MARTIN, SJ ;
RIMA, BK .
VIRUS RESEARCH, 1989, 12 (01) :61-75
[5]   Genetic heterogeneity of mumps virus in the United Kingdom: Identification of two new genotypes [J].
Jin, L ;
Beard, S ;
Brown, DWG .
JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (03) :829-833
[6]   Sequence analysis of F, SH, and HN genes among mumps virus strains in Japan [J].
Kashiwagi, Y ;
Takami, T ;
Mori, T ;
Nakayama, T .
ARCHIVES OF VIROLOGY, 1999, 144 (03) :593-599
[7]   DIFFERENTIATION OF VACCINE AND WILD MUMPS VIRUSES BY POLYMERASE CHAIN-REACTION AND NUCLEOTIDE SEQUENCING OF THE SH GENE - BRIEF REPORT [J].
KUNKEL, U ;
DRIESEL, G ;
HENNING, U ;
GERIKE, E ;
WILLERS, H ;
SCHREIER, E .
JOURNAL OF MEDICAL VIROLOGY, 1995, 45 (02) :121-126
[8]  
*MIN HLTH WELF KOR, 1998, YB HLTH WELF STAT KO, P42
[9]   Characterization of five conserved genotypes of the mumps virus small hydrophobic (SH) protein gene [J].
Orvell, C ;
Kalantari, M ;
Johansson, B .
JOURNAL OF GENERAL VIROLOGY, 1997, 78 :91-95
[10]  
PLOTKIN SA, 1994, VACCINES, P277