The onset of germ cell migration in the mouse embryo

被引：214

作者：

Anderson, R

Copeland, TK

Schöler, H

Heasman, J

Wylie, C

机构：

[1] Univ Minnesota, Sch Med, Dept Pediat, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Sch Med, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Sch Med, Mol Cellular Dev Biol & Genet Program, Minneapolis, MN 55455 USA

[4] European Mol Biol Lab, Gene Express Programme, D-69012 Heidelberg, Germany

来源：

MECHANISMS OF DEVELOPMENT | 2000年 / 91卷 / 1-2期

关键词：

alkaline phosphatase; allantois; cell migration; filopodia; FM4-64; germline; green fluorescent protein; hind-gut; mouse-murine development; Oct4; primitive streak; primordial germ cell; SSEA-I; time-lapse confocal microscopy;

D O I：

10.1016/S0925-4773(99)00271-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mouse primordial germ cells (PGCs) are specified between embryonic day 6.5 (E6.5) and E7.5, when they have been visualized as an alkaline phosphatase-positive (AP+) cell population in the developing allantois. By E8.5, they are embedded in the hind-gut epithelium. Previous experiments have suggested different sites for PGCs' origin, and it is unclear how they reach the gut epithelium. We have used transgenic mice expressing GFP under a truncated Oct4 promoter to visualize living PGCs. We find GFP+/AP+ cells in the posterior end of the primitive streak as a dispersed population of cells actively migrating into the allantois, and directly into the adjacent embryonic endoderm. Time-lapse analysis shows these cells to be actively migratory from the time they exit the primitive streak. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：61 / 68

页数：8

共 25 条

[1]

Anderson R, 1999, DEVELOPMENT, V126, P1655

[2]

Anderson R, 1999, J REPROD FERTIL, V116, P379, DOI 10.1530/jrf.0.1160379

[3]

[Anonymous], 1994, MANIPULATING MOUSE E

[4] The origin and migration of primordial germ cells in the mouse [J].