Binding sites for melanin-concentrating hormone in the human brain

被引：26

作者：

Sone, M

Takahashi, K ^{[1
]}

Murakami, O

Totsune, K

Arihara, Z

Satoh, F

Sasano, H

Ito, H

Mouri, T

机构：

[1] Tohoku Univ, Sch Med, Dept Mol Biol & Appl Physiol, Sendai, Miyagi 9808575, Japan

[2] Natl Iwate Hosp, Dept Internal Med, Ichinoseki, Iwate 0210015, Japan

[3] Tohoku Univ, Sch Med, Dept Internal Med 2, Sendai, Miyagi 9808574, Japan

[4] Tohoku Univ, Sch Med, Dept Pathol, Sendai, Miyagi 9808575, Japan

[5] Mouri Clin, Div Neuroendocrinol, Natori, Miyagi 9811224, Japan

来源：

PEPTIDES | 2000年 / 21卷 / 02期

关键词：

melanin-concentrating hormone; receptor; human brain; melanocortin;

D O I：

10.1016/S0196-9781(99)00206-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding sites for melanin-concentrating hormone (MCH) in human brain were investigated and characterized by radioligand binding Specific binding sites for MCH were present in every region of human brain (cerebral cortex, cerebellum, thalamus, hypothalamus, pens, and medulla oblongata) obtained at autopsy. alpha-Melanocyte stimulating hormone or ACTH was a Door inhibitor of I-125-MCH binding (IC50 > 1 mu M) compared with MCH (IC50 = 0.3 +/- 0.07 nM, mean +/- SEM, n = 3). Scatchard plots of I-125-MCH binding in human brain (thalamus) gave a dissociation constant of 0.2 +/- 0.06 nM and maximal binding of 5.8 +/- 0.3 fmol/mg protein (n = 3). These findings suggest that specific MCH binding sites that differ from the melanocortin receptors exist in human brain. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：245 / 250

页数：6

共 34 条

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