Alcohol-oxidation activity of whole cells of Pichia pastoris entrapped in hybrid gels composed of Ca-alginate and organic silicate

被引：16

作者：

Kawakami, K

Furukawa, SY

机构：

[1] Department of Chemical Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1997年 / 67卷 / 1-2期

关键词：

sol-gel entrapment; alginate; silicate; hybrid gel; immobilized whole cells; Pichia pastoris; oxidation; benzyl alcohol; organic solvent;

D O I：

10.1007/BF02787838

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Formation of hybrid gels from an aqueous mixture of alginate and alkoxysilanes has been applied to immobilization of whole cells of Pichia pastoris catalyzing oxidation of benzyl alcohol in organic solvent. The amount of benzaldehyde produced after a prolonged reaction period was 1.2 and 1.8 times greater with the hybrid gels of alginate + silicate and alginate + methyl-substituted silicate, respectively, than with the alginate single gel. This was ascribed to a facilitated release rate of aldehyde, which acted as a strong inhibitor against the enzyme alcohol oxidase, from the inside of the cells to organic medium through hydrophobic gel matrix.

引用

页码：23 / 31

页数：9

共 7 条

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