Evidence for a role of C-terminal amino acid residues in skeletal muscle Ca2+ release channel (ryanodine receptor) function

The effects of deleting 1, 3 and 15 amino acid residues from the highly conserved C-terminus of the tetrameric skeletal muscle ryanodine receptor (RyR) complex were determined. Immunoblot analysis indicated similar expression levels in HEK293 cells for full-length and mutant proteins. Fun-length and RyR lacking the last amino acid showed (IH)-H-3]ryanodine binding and single channel activities typical of native receptors. Deletion of 3 amino acids resulted in decreased activities, whereas deletion of 15 amino acids yielded an inactive RyR. These results suggest that the most 15 C-terminal amino acids are important for the expression of a functional RyR complex. (C) 1997 Federation of European Biochemical Societies.