Dietary protein regulates in vitro lipogenesis and lipogenic gene expression in broilers

被引:44
作者
Rosebrough, RW [1 ]
Poch, SM [1 ]
Russell, BA [1 ]
Richards, MP [1 ]
机构
[1] ARS, Growth Biol Lab, Anim & Nat Resources Inst, USDA,Beltsville Agr Res Ctr, Beltsville, MD 20705 USA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2002年 / 132卷 / 02期
关键词
gene expression; diets; metabolism; chickens; lipogenesis; enzyme activities;
D O I
10.1016/S1095-6433(02)00084-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this experiment was to determine the possible relationship between certain indices of lipid metabolism and specific gene expression in chickens fed graded levels of dietary crude protein. Male, broiler chickens growing from 7 to 28 days of age were fed diets containing 12, 21 or 30% protein ad libitum. In addition, another group of birds was fed on a regimen consisting of a daily change in the dietary protein level (12 or 30%). This latter group was further subdivided such that one-half of the birds received each level of protein on alternating days. Birds were sampled from 28 to 30 days of age. Measurements taken included in vitro lipogenesis, malic enzyme activity the expression of the genes for malic enzyme, fatty acid synthase and acetyl coenzyme carboxylase. In vitro lipogenesis and malic enzyme activity were inversely related to dietary protein levels (12-30%) and to acute changes from 12 to 30%. In contrast, expression of malic enzyme, fatty acid synthase and acetyl CoA carboxylase genes were constant over a dietary protein range of 12-21%, but decreased by feeding a 30% protein diet (acute or chronic feeding). Results of the present study demonstrate a continued role for protein in the regulation of broiler metabolism. It should be pointed out, however, that metabolic regulation at the gene level only occurs when feeding very high levels of dietary protein. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:423 / 431
页数:9
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