The Plasmodium falciparum 6 kb element is polycistronically transcribed

被引:32
作者
Ji, YE
Mericle, BL
Rehkopf, DH
Anderson, JD
Feagin, JE
机构
[1] SEATTLE BIOMED RES INST,SEATTLE,WA 98109
[2] UNIV WASHINGTON,DEPT PATHOBIOL,SCH PUBL HLTH & COMMUNITY MED,SEATTLE,WA 98195
关键词
plasmodium falciparum; mitochondrial genes; rRNA; polycistronic transcription;
D O I
10.1016/0166-6851(96)02712-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Plasmodium falciparum 6 kb element encodes three protein coding genes and highly fragmented large and small subunit rRNAs; its gene content makes it the probable mitochondrial genome. Many of the genes are encoded so close to each other that there is insufficient room for specific promoters upstream of each gene, RNase protection analysis of two rRNA fragments whose genes are adjacent provided evidence for a polycistronic transcript containing sequences from both, as well as separate small RNAs. To evaluate the possibility of further polycistronic transcription, several sets of oligonucleotide primers located in different regions of the 6 kb element were employed to amplify cDNAs. These analyses have revealed the existence of 6 kb element transcripts as long as 5.9 kb. Both mRNA and rRNA sequences are included on these putative precursor transcripts. Since these types of RNA are known to have different patterns of abundance changes during the erythrocytic portion of the parasite lifecycle, RNA stability is presumably an important feature in regulating mitochondrial transcript abundance.
引用
收藏
页码:211 / 223
页数:13
相关论文
共 33 条
[1]   SEQUENCE IDENTIFICATION OF CYTOCHROME-B IN PLASMODIUM-GALLINACEUM [J].
ALDRITT, SM ;
JOSEPH, JT ;
WIRTH, DF .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (09) :3614-3620
[2]  
[Anonymous], 1989, SYNTHETIC OLIGONUCLE
[3]   SCRAMBLED RIBOSOMAL-RNA GENE PIECES IN CHLAMYDOMONAS REINHARDTII MITOCHONDRIAL-DNA [J].
BOER, PH ;
GRAY, MW .
CELL, 1988, 55 (03) :399-411
[4]   THE EXCISION OF INTERVENING SEQUENCES FROM SALMONELLA-23S RIBOSOMAL-RNA [J].
BURGIN, AB ;
PARODOS, K ;
LANE, DJ ;
PACE, NR .
CELL, 1990, 60 (03) :405-414
[5]   PRECISE IDENTIFICATION OF CLEAVAGE SITES INVOLVED IN THE UNUSUAL PROCESSING OF TRYPANOSOME RIBOSOMAL-RNA [J].
CAMPBELL, DA ;
KUBO, K ;
CLARK, CG ;
BOOTHROYD, JC .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (01) :113-124
[6]   INVITRO TRANSCRIPTION OF HUMAN MITOCHONDRIAL-DNA - ACCURATE TERMINATION REQUIRES A REGION OF DNA-SEQUENCE THAT CAN FUNCTION BIDIRECTIONALLY [J].
CHRISTIANSON, TW ;
CLAYTON, DA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) :6277-6281
[7]  
CLAYTON DA, 1984, ANNU REV BIOCHEM, V53, P573
[8]   THE SINGLE GROUP-I INTRON IN THE CHLOROPLAST RRNL GENE OF CHLAMYDOMONAS-HUMICOLA ENCODES A SITE-SPECIFIC DNA ENDONUCLEASE (I-CHUI) [J].
COTE, V ;
MERCIER, JP ;
LEMIEUX, C ;
TURMEL, M .
GENE, 1993, 129 (01) :69-76
[9]   HOMOLOGIES BETWEEN THE CONTIGUOUS AND FRAGMENTED RIBOSOMAL-RNAS OF THE 2 PLASMODIUM-FALCIPARUM EXTRACHROMOSOMAL DNAS ARE LIMITED TO CORE SEQUENCES [J].
FEAGIN, JE ;
WERNER, E ;
GARDNER, MJ ;
WILLIAMSON, DH ;
WILSON, RJM .
NUCLEIC ACIDS RESEARCH, 1992, 20 (04) :879-887
[10]   APOCYTOCHROME-B AND OTHER MITOCHONDRIAL-DNA SEQUENCES ARE DIFFERENTIALLY EXPRESSED DURING THE LIFE-CYCLE OF TRYPANOSOMA-BRUCEI [J].
FEAGIN, JE ;
JASMER, DP ;
STUART, K .
NUCLEIC ACIDS RESEARCH, 1985, 13 (12) :4577-4596