Polyvinyl alcohol-glutaraldehyde network as a support for protein immobilisation

被引：35

作者：

Araujo, AM

Neves, MT

Azevedo, WM

Oliveira, GG

Ferreira, DL

Coelho, RAL

Figueiredo, EAP

Carvalho, LB

机构：

[1] UNIV FED PERNAMBUCO,LAB IMUNOPATOL KEIZO ASAMI,BR-50670910 RECIFE,PE,BRAZIL

[2] HEMOPE FUNDACAO HEMATOL & HEMOTERAPIA PERNAMBUCO,RECIFE,PE,BRAZIL

[3] UNIV FED PERNAMBUCO,DEPT QUIM FUNDAMENTAL,BR-50670910 RECIFE,PE,BRAZIL

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 02期

关键词：

D O I：

10.1023/A:1018404103626

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyvinyl alcohol-glutaraldehyde (PVA-glut) network was synthesised in bead and disc forms and used for protein immobilisation. Xanthine oxidase, alpha-amylase and amyloglucosidase were covalently fixed on the beads yielding preparations with specific activities retention of 72.3%, 1.6% and 1.4%, respectively. Km of xanthine oxidase PVA-glut beads (24+/-4 mu M) was slightly higher than that estimated for the soluble enzyme (16+/-2 mu M). Antigens from Schistosoma mansoni were covalently fixed onto PVA-glut discs and ELISA was carried out. The relationship between the amount of fixed antigen and the results obtained by ELISA showed a hyperbolic curve and the saturation of antigen-antibody complex was achieved at the plateau.

引用

页码：67 / 70

页数：4

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