Determination of reticulo-rumen and whole-stomach digestion in lactating cows by omasal canal or duodenal sampling

被引:147
作者
Ahvenjärvi, S [1 ]
Vanhatalo, A [1 ]
Huhtanen, P [1 ]
Varvikko, T [1 ]
机构
[1] Agr Res Ctr, MTT, FIN-31600 Jokioinen, Finland
关键词
ruminant; digestibility; markers; omasum;
D O I
10.1017/S0007114500000106
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Four ruminally and duodenally cannulated multiparous Finnish Ayrshire cows were fed on diets consisting of grass silage (0.6 kg/kg DM) and one of four concentrates: barley, barley+urea, barley+rapeseed meal and barley+rapeseed cake. The objective of the present study was to compare omasal canal and duodenal digesta flows. Values for digesta flow into the omasal canal and duodenum were determined using a triple-marker method based on Co-EDTA, Yb-acetate and indigestible neutral-detergent fibre (NDF) markers. Microbial non-NH3 N (NAN) flow was assessed by purine flow. Microbial samples to determine the bacterial purine:N ratio were harvested from the rumen, omasum and duodenum. Organic matter flow was significantly lower into the omasum than the duodenum, indicating an endogenous organic matter secretion into the abomasum. In contrast, NDF and acid-detergent fibre flows were significantly higher into the omasum indicating digestion of fibre in the omasum. Microbial NAN flows were significantly different (P < 0.001) when estimates were based on bacterial samples harvested from different sites. Differences in total NAN, microbial NAN and dietary NAN flows entering the omasal canal and duodenum were non-significant. The results indicated that the omasal sampling technique provides a promising alternative to the duodenal sampling technique to investigate forestomach digestion in dairy cows and offers an alternative means to study rumen N metabolism.
引用
收藏
页码:67 / 77
页数:11
相关论文
共 54 条
[1]   Effects of supplementation of a grass silage and barley diet with urea, rapeseed meal and heat-moisture-treated rapeseed cake on omasal digesta flow and milk production in lactating dairy cows [J].
Ahvenjärvi, S ;
Vanhatalo, A ;
Huhtanen, P ;
Varvikko, T .
ACTA AGRICULTURAE SCANDINAVICA SECTION A-ANIMAL SCIENCE, 1999, 49 (03) :179-189
[2]  
Barker SB, 1941, J BIOL CHEM, V138, P535
[3]   COMPOSITION OF RUMINAL BACTERIA HARVESTED FROM STEERS AS INFLUENCED BY DIETARY ENERGY-LEVEL, FEEDING FREQUENCY, AND ISOLATION TECHNIQUES [J].
CECAVA, MJ ;
MERCHEN, NR ;
GAY, LC ;
BERGER, LL .
JOURNAL OF DAIRY SCIENCE, 1990, 73 (09) :2480-2488
[4]   QUANTITATION OF MICROORGANISMS ASSOCIATED WITH THE PARTICULATE PHASE OF RUMINAL INGESTA [J].
CRAIG, WM ;
BRODERICK, GA ;
RICKER, DB .
JOURNAL OF NUTRITION, 1987, 117 (01) :56-62
[5]  
DOBSON DE, 1984, J BIOL CHEM, V259, P1607
[6]  
ENGELHARDT WV, 1975, DIGESTION METABOLISM, P217
[7]  
Faichney G., 1975, DIGESTION METABOLISM, V1, P277
[8]  
Faichney G. G., 1993, P53
[9]   EFFECT OF FORMALDEHYDE TREATMENT OF A CONCENTRATE DIET ON PASSAGE OF SOLUTE AND PARTICLE MARKERS THROUGH GASTROINTESTINAL-TRACT OF SHEEP [J].
FAICHNEY, GJ .
AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH, 1975, 26 (02) :319-327
[10]   Effects of concentrates in a hay diet on the contribution of anaerobic fungi, protozoa and bacteria to nitrogen in rumen and duodenal digesta in sheep [J].
Faichney, GJ ;
Poncet, C ;
Lassalas, B ;
Jouany, JP ;
Millet, L ;
Dore, J ;
Brownlee, AG .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 1997, 64 (2-4) :193-213