Changes in actin dynamics are involved in salicylic acid signaling pathway

被引：21

作者：

Matouskova, Jindriska ^{[1
,4
]}

Janda, Martin ^{[1
]}

Fiser, Radovan ^{[2
]}

Sasek, Vladimir ^{[3
]}

Kocourkova, Daniela ^{[3
]}

Burketova, Lenka ^{[3
]}

Duskova, Jirina ^{[4
]}

Martinec, Jan ^{[3
]}

Valentova, Olga ^{[1
]}

机构：

[1] Prague Inst Chem Technol, Dept Biochem & Microbiol, CR-16628 Prague 6, Czech Republic

[2] Charles Univ Prague, Fac Sci, Prague 12800 2, Czech Republic

[3] Acad Sci Czech Republ, Inst Expt Bot, CR-16502 Prague 6, Lysolaje, Czech Republic

[4] Charles Univ Prague, Fac Pharm Hradec Kralove, Hradec Kralove 50005, Czech Republic

来源：

PLANT SCIENCE | 2014年 / 223卷

关键词：

Actin dynamics; Salicylic acid; PR genes; Phosphatidic acid; PHOSPHATIDIC-ACID; PHOSPHOLIPASE-D; TRANSCRIPTION FACTORS; DEFENSE-RESPONSES; BINDING PROTEINS; CAPPING PROTEIN; LATRUNCULIN-B; RAPID GROWTH; ARABIDOPSIS; CELLS;

D O I：

10.1016/j.plantsci.2014.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in actin cytoskeleton dynamics are one of the crucial players in many physiological as well as non-physiological processes in plant cells. Positioning of actin filament arrays is necessary for successful establishment of primary lines of defense toward pathogen attack, depolymerization leads very often to the enhanced susceptibility to the invading pathogen. On the other hand it was also shown that the disruption of actin cytoskeleton leads to the induction of defense response leading to the expression of PATHOGENESIS RELATED proteins (PR). In this study we show that pharmacological actin depolymerization leads to the specific induction of genes in salicylic acid pathway but not that involved in jasmonic acid signaling. Life imaging of leafs of Arabidopsis thaliana with GFP-tagged fimbrin (GFP-fABD2) treated with 1 mM salicylic acid revealed rapid disruption of actin filaments resembling the pattern viewed after treatment with 200 nM latrunculin B. The effect of salicylic acid on actin filament fragmentation was prevented by exogenous addition of phosphatidic acid, which binds to the capping protein and thus promotes actin polymerization. The quantitative evaluation of actin filament dynamics is also presented. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：36 / 44

页数：9

共 42 条

[1] A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection [J].

Bretz, JR ;

Mock, NM ;

Charity, JC ;

Zeyad, S ;

Baker, CJ ;

Hutcheson, SW .

MOLECULAR MICROBIOLOGY, 2003, 49 (02) :389-400

[2] Large-scale identification of tubulin-binding proteins provides insight on subcellular trafficking, metabolic channeling, and signaling in plant cells [J].

Chuong, SDX ;

Good, AG ;

Taylor, GJ ;

Freeman, MC ;

Moorhead, GBG ;

Muench, DG .

MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (10) :970-983

[3] Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response [J].

Clay, Nicole K. ;

Adio, Adewale M. ;

Denoux, Carine ;

Jander, Georg ;

Ausubel, Frederick M. .

SCIENCE, 2009, 323 (5910) :95-101

[4] Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis [J].

Czechowski, T ;

Stitt, M ;

Altmann, T ;

Udvardi, MK ;

Scheible, WR .

PLANT PHYSIOLOGY, 2005, 139 (01) :5-17

[5] The arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors [J].

Després, C ;

DeLong, C ;

Glaze, S ;

Liu, E ;

Fobert, PR .

PLANT CELL, 2000, 12 (02) :279-290

[6] Wide-ranging effects of eight cytochalasins and latrunculin A and B on intracellular motility and actin filament reorganization in characean internodal cells [J].

Foissner, Ilse ;

Wasteneys, Geoffrey O. .

PLANT AND CELL PHYSIOLOGY, 2007, 48 (04) :585-597

[7]

Gibbon B.C., 1999, PLANT CELL, V11, P1363

[8] Rapid and dynamic subcellular reorganization following mechanical stimulation of Arabidopsis epidermal cells mimics responses to fungal and oomycete attack [J].

Hardham, Adrienne R. ;

Takemoto, Daigo ;

White, Rosemary G. .

BMC PLANT BIOLOGY, 2008, 8 (1)

[9] The Plant Actin Cytoskeleton Responds to Signals from Microbe-Associated Molecular Patterns [J].

Henty-Ridilla, Jessica L. ;

Shimono, Masaki ;

Li, Jiejie ;

Chang, Jeff H. ;

Day, Brad ;

Staiger, Christopher J. .

PLOS PATHOGENS, 2013, 9 (04)

[10] Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants [J].

Higaki, Takumi ;

Kurusu, Takamitsu ;

Hasezawa, Seiichiro ;

Kuchitsu, Kazuyuki .

JOURNAL OF PLANT RESEARCH, 2011, 124 (03) :315-324

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