Evaluation of different techniques for washing cats: Quantitation of allergen removed from the cat and the effect on airborne Fel d 1

Background and Objective: The purpose of this study was to examine the quantity and distribution of the major cat allergen, Fel d 1, on cats and to evaluate the efficacy of washing, both in removing allergen from the cat and reducing airborne allergen levels. Methods: Airborne samples were collected on four glass fiber filters in a 30 m(3) room, before and 3 hours after serial washing of eight cats (45-minute sampling at 18 L/min for each filter). Aliquots of hair and bath water were also collected and assayed for Eel d 1 content. Results: Extracting cat hair with tap water or pet shampoo for 3 minutes removed mean levels of 191 and 245 mu g of Fel d 1 per gram of hair, respectively; the quantity of allergen on samples of cat hair ranged from 1 mu g/gm to more than 1770 mu g/gm. The highest concentration of allergen was found on hair from the neck Estimates of the total Eel d I on the cat, based on shaving the whole cat, ranged from 3 to 142 mg (mean = 67 mg). Washing cats reduced airborne allergen 3 hours later. Washing three cats at weekly intervals for 5 weeks in a veterinarian's office produced a mean decrease of 44% in airborne Eel d 1 (n = 15, p < 0.02). Washing three cats by immersion for 3 minutes at weekly intervals for a 1-month period produced a mean decrease in airborne allergen of 79% (n = 12, p < 0.001). However, after repeated washing, the airborne levels before the next wash were not consistently decreased. The quantity of Fel d 1 removed by immersion varied from 1 to 35 mg. Conclusion: Cats carry large quantities of Fel d 1, only a small proportion of which (similar to 0.002%/hr) becomes airborne. Washing cats by immersion will remove significant allergen from the cat and can reduce the quantity of Eel d 1 becoming airborne, However, the decrease is not maintained at 1 week.