Multiple mitochondrial DNA polymerases in Trypanosoma brucei

被引：111

作者：

Klingbeil, MM ^{[1
]}

Motyka, SA ^{[1
]}

Englund, PT ^{[1
]}

机构：

[1] Johns Hopkins Med Sch, Dept Biol Chem, Baltimore, MD 21205 USA

来源：

MOLECULAR CELL | 2002年 / 10卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(02)00571-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetoplast DNA (kDNA), the unusual mitochondrial DNA of Trypanosoma brucei, is a network containing thousands of catenated circles. Database searching for a kDNA replicative polymerase (pol) revealed no mitochondrial pol gamma homolog. Instead, we identified four proteins (TbPOLIA, IB, IC, and ID) related to bacterial pol 1. Remarkably, all four localized to the mitochondrion. TbPOLIB and TbPOLIC localized beside the kDNA where replication occurs, and their knockdown by RNA interference caused kDNA network shrinkage. Furthermore, silencing of TbPOILIC caused loss of both minicircles and maxicircles and accumulation of minicircle replication intermediates, consistent with a role in replication. While typical mitochondria contain one DNA polymerase, pol gamma, trypanosome mitochondria contain five such enzymes, including the previously characterized pol beta.

引用

页码：175 / 186

页数：12

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