Network pharmacology analyses of the antithrombotic pharmacological mechanism of Fufang Xueshuantong Capsule with experimental support using disseminated intravascular coagulation rats

被引：159

作者：

Sheng, Shujing ^{[1
,2
]}

Wang, Jinxu ^{[1
]}

Wang, Lirong ^{[3
]}

Liu, Hong ^{[1
]}

Li, Peibo ^{[1
]}

Liu, Menghua ^{[1
]}

Long, Chaofeng ^{[2
]}

Xie, Chengshi ^{[2
]}

Xie, Xiangqun ^{[3
]}

Su, Weiwei ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Guangzhou Qual R&D Ctr Tradit Chinese Med, Sch Life Sci, Guangdong Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China

[2] Guangdong Zhongsheng Pharmaceut Co Ltd, Dongguan 523325, Peoples R China

[3] Univ Pittsburgh, Drug Discovery Inst, Sch Pharm, Computat Chem Genom Screening Ctr,Dept Pharmaceut, Pittsburgh, PA 15261 USA

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2014年 / 154卷 / 03期

关键词：

Fufang Xueshuantong Capsule; Network pharmacology; Thrombosis disease; Disseminated intravascular coagulation; MOLECULAR-WEIGHT HEPARIN; THROMBOSIS; PROTEIN; MODEL; ACTIVATION; INHIBITION; PREDICTION; GUIDELINES; MANAGEMENT; PHYSIOLOGY;

D O I：

10.1016/j.jep.2014.04.048

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: Fufang Xueshuantong (FXST) Capsule is developed on a traditional Chinese medicine remedy, with a four-herb formula of Panax notoginseng, Radix astragali, Salvia miltiorrhizae and Radix scrophulariaceae. It has been used for treatment of the clinic cardiovascular disease for many years. Materials and Methods: Due to its complexity of compositions and polypharmacological effects, it often complicates understanding of the mechanisms of action. In the present work, we have constructed an integrated model of system pharmacology to investigate the polypharmacological mechanisms of FXST formulation for treatment of thrombosis disease. Results: The predicted results showed that 22 ingredients in FXST were closely associated with 41 protein targets related to blood coagulation, fibrinolysis and platelet aggregation. Through analysis of the compound-protein target association, significant cross-targets between each herb indicated the multiple active chemical ingredients might interact with the same target simultaneously and thus explained the synergistic mechanisms of the principle of Traditional Chinese medicines (TCMs) as "Jun (emperor) - Chen (minister) - Zuo (adjuvant) - Shi (courier)". To validate the polypharmacological effects predicted by our network pharmacology (NetPharm) analysis, we have carried out experimental investigation the effects of FXST on the disorders of the blood coagulation system in a lipopolysaccharide-induced disseminated intravascular coagulation (DIC) rat model. The results showed that FXST could significantly ameliorate the activation of coagulation system, which is congruent with the cross-target prediction by NetPharm approach. Conclusions: The combined investigations provide more insight into better understanding of the pharmacological mechanisms of FXST, and may also offer an alternative avenue to further explore the chemical and pharmacological basis of TCMs. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：735 / 744

页数：10

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