Western Blotting using Chemiluminescent Substrates

被引：16

作者：

Alegria-Schaffer, Alice ^{[1
]}

机构：

[1] Thermo Fisher Sci, Rockford, IL 61101 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT C | 2014年 / 541卷

关键词：

D O I：

10.1016/B978-0-12-420119-4.00019-7

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture (Towbin et al., 1979). The technique enables indirect detection of protein samples immobilized on a nitrocellulose or polyvinylidene fluoride (PVDF) membrane.

引用

页码：251 / 259

页数：9

共 2 条

[1]

PERFORMING AND OPTIMIZING WESTERN BLOTS WITH AN EMPHASIS ON CHEMILUMINESCENT DETECTION [J].