Determination of phosphorylation sites in peptides and proteins employing a volatile Edman reagent

被引：3

作者：

Fischer, WH ^{[1
]}

Hoeger, CA ^{[1
]}

Meisenhelder, J ^{[1
]}

Hunter, T ^{[1
]}

Craig, AG ^{[1
]}

机构：

[1] SALK INST BIOL STUDIES, MOL BIOL & VIROL LAB, LA JOLLA, CA 92037 USA

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1997年 / 16卷 / 05期

关键词：

manual Edman degradation; phosphoprotein analysis; trifluoroethyl isothiocyanate;

D O I：

10.1023/A:1026368201651

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A manual Edman degradation protocol has been developed that allows the identification of phosphorylation sites in P-32-labeled peptides at the subpicomole level. By using both a volatile reagent, trifluoroethyl isothiocyanate, and volatile buffers, extraction steps are rendered unnecessary and cycle times can be reduced to 45 min. The protocol was employed to identify the site of phosphorylation in phosphoserine- and phosphotyrosine-containing peptides.

引用

页码：329 / 334

页数：6

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