Characterization of acid-tolerant H2/CO2-utilizing methanogenic enrichment cultures from an acidic peat bog in New York State

被引:41
作者
Brauer, Suzanna L.
Yashiro, Erika
Ueno, Norikiyo G.
Yavitt, Joseph B.
Zinder, Stephen H.
机构
[1] Cornell Univ, Dept Microbiol, Ithaca, NY 14853 USA
[2] Cornell Univ, Dept Nat Resources, Ithaca, NY 14853 USA
关键词
methanogenesis; acidiphiles; Methanomicrobiales; peatlands; acidic bogs; bacteria;
D O I
10.1111/j.1574-6941.2006.00107.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two methanogenic cultures were enriched from acidic peat soil using a growth medium buffered to c. pH 5. One culture, 6A, was obtained from peat after incubation with H-2/CO2, whereas culture NTA was derived from a 10(-4) dilution of untreated peat into a modified medium. 16S rRNA gene clone libraries from each culture contained one methanogen and two bacterial sequences. The methanogen 16S rRNA gene sequences were 99% identical with each other and belonged to the novel 'R-10/Fen cluster' family of the Methanomicrobiales, whereas their mcrA sequences were 96% identical. One bacterial 16S rRNA gene sequence from culture 6A belonged to the Bacteroidetes and showed 99% identity with sequences from methanogenic enrichments from German and Russian bogs. The other sequence belonged to the Firmicutes and was identical to a thick rod-shaped citrate-utilizing organism isolated from culture 6A, the numbers of which decreased when the Ti (III) chelator was switched from citrate to nitrilotriacetate. Bacterial clones from the NTA culture clustered in the Delta- and Betaproteobacteria. Both cultures contained thin rods, presumably the methanogens, as the predominant morphotype, and represent a significant advance in characterization of the novel acidiphilic R-10 family methanogens.
引用
收藏
页码:206 / 216
页数:11
相关论文
共 31 条
[1]   METHANOGENS - RE-EVALUATION OF A UNIQUE BIOLOGICAL GROUP [J].
BALCH, WE ;
FOX, GE ;
MAGRUM, LJ ;
WOESE, CR ;
WOLFE, RS .
MICROBIOLOGICAL REVIEWS, 1979, 43 (02) :260-296
[2]   Methane biogeochemistry and methanogen communities in two northern peatland ecosystems, New York State [J].
Basiliko, N ;
Yavitt, JB ;
Dees, PM ;
Merkel, SM .
GEOMICROBIOLOGY JOURNAL, 2003, 20 (06) :563-577
[3]   Desultovibrio putealis sp nov., a novel sulfate-reducing bacterium isolated from a deep subsurface aquifer [J].
Basso, O ;
Caumette, P ;
Magot, M .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2005, 55 :101-104
[4]   Methanogenesis in McLean Bog, an acidic peat bog in upstate New York:: Stimulation by H2/CO2 in the presence of rifampicin, or by low concentrations of acetate [J].
Bräuer, SL ;
Yavitt, JB ;
Zinder, SH .
GEOMICROBIOLOGY JOURNAL, 2004, 21 (07) :433-443
[5]   BIOGEOCHEMICAL ASPECTS OF ATMOSPHERIC METHANE [J].
Cicerone, R. ;
Oremland, R. .
GLOBAL BIOGEOCHEMICAL CYCLES, 1988, 2 (04) :299-327
[6]   ESTIMATION OF BACTERIAL DENSITIES BY MEANS OF THE MOST PROBABLE NUMBER [J].
COCHRAN, WG .
BIOMETRICS, 1950, 6 (02) :105-116
[7]   Characterization of a highly enriched Dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene [J].
Duhamel, M ;
Mo, K ;
Edwards, EA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (09) :5538-5545
[8]  
Felsenstein J., 2004, PHYLIP VERSION 3 6
[9]   Enrichment culture and microscopy conceal diverse thermophilic Synechococcus populations in a single hot spring microbial mat habitat [J].
Ferris, MJ ;
RuffRoberts, AL ;
Kopczynski, ED ;
Bateson, MM ;
Ward, DM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (03) :1045-1050
[10]   Microsite-dependent changes in methanogenic populations in a boreal oligotrophic fen [J].
Galand, PE ;
Fritze, H ;
Yrjälä, K .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (11) :1133-1143