Occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: Microbiological findings

被引:42
作者
Giuliana, G
Ammatuna, P
Pizzo, G
Capone, F
DAngelo, M
机构
[1] UNIV PALERMO,DEPT PERIODONTOL,I-90133 PALERMO,ITALY
[2] UNIV PALERMO,DEPT HYG & MICROBIOL,I-90133 PALERMO,ITALY
关键词
invading bacteria; radicular dentin; periodontal pathogens; periodontal disease;
D O I
10.1111/j.1600-051X.1997.tb00215.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. The purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. Samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. Dentin samples were cultured anaerobically. The chosen methodology allowed the determination of the numbers of bacteria present in both deeper and outer part of dentinal tubules, and the bacterial concentration in dentin samples, expressed as colony forming units per mg of tissue (CFU/mg). Invading bacteria was detected in 14 (53.8%) samples from periodontally diseased teeth. The bacterial concentration ranged from 831.84 to 11971.3 CFU/mg (mean+/-standard deviation: 3043.15+/-2763.13). Micro-organisms identified included putative periodontal pathogens such as Prevotella intermedia, Porphyro-monas gingivalis, Fusobacterium nucleatum, Bacteroides forsythus, Peptostreptococcus micros and Streptococcus intermedius. These findings suggest that radicular dentin could act as bacterial reservoir from which periodontal pathogens can recolonize treated periodontal pockets, contributing to the failure of therapy and recurrence of disease.
引用
收藏
页码:478 / 485
页数:8
相关论文
共 66 条
[1]  
ADRIAENS PA, 1987, J DENT RES, V66, P338
[2]   BACTERIAL INVASION IN ROOT CEMENTUM AND RADICULAR DENTIN OF PERIODONTALLY DISEASED TEETH IN HUMANS - A RESERVOIR OF PERIODONTOPATHIC BACTERIA [J].
ADRIAENS, PA ;
DEBOEVER, JA ;
LOESCHE, WJ .
JOURNAL OF PERIODONTOLOGY, 1988, 59 (04) :222-230
[3]   ULTRASTRUCTURAL OBSERVATIONS ON BACTERIAL INVASION IN CEMENTUM AND RADICULAR DENTIN OF PERIODONTALLY DISEASED HUMAN-TEETH [J].
ADRIAENS, PA ;
EDWARDS, CA ;
DEBOEVER, JA ;
LOESCHE, WJ .
JOURNAL OF PERIODONTOLOGY, 1988, 59 (08) :493-503
[4]  
ADRIAENS PA, 1984, CARIES RES, V18, P160
[5]   RECOVERY OF A-ACTINOMYCETEMCOMITANS FROM TEETH, TONGUE, AND SALIVA [J].
ASIKAINEN, S ;
ALALUUSUA, S ;
SAXEN, L .
JOURNAL OF PERIODONTOLOGY, 1991, 62 (03) :203-206
[6]  
Borghetti A, 1987, Int J Periodontics Restorative Dent, V7, P23
[7]   SCANNING AND TRANSMISSION ELECTRON-MICROSCOPIC STUDY OF TISSUE-INVADING MICROORGANISMS IN LOCALIZED JUVENILE PERIODONTITIS [J].
CARRANZA, FA ;
SAGLIE, R ;
NEWMAN, MG ;
VALENTIN, PL .
JOURNAL OF PERIODONTOLOGY, 1983, 54 (10) :598-617
[8]   TISSUE LOCALIZATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IN HUMAN PERIODONTITIS .1. LIGHT, IMMUNOFLUORESCENCE AND ELECTRON-MICROSCOPIC STUDIES [J].
CHRISTERSSON, LA ;
ALBINI, B ;
ZAMBON, JJ ;
WIKESJO, UME ;
GENCO, RJ .
JOURNAL OF PERIODONTOLOGY, 1987, 58 (08) :529-539
[9]   TOPICAL APPLICATION OF TETRACYCLINE-HCL IN HUMAN PERIODONTITIS [J].
CHRISTERSSON, LA ;
NORDERYD, OM ;
PUCHALSKY, CS .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1993, 20 (02) :88-95
[10]   A QUANTITATIVE STUDY OF CEMENTUM REMOVAL WITH HAND CURETTES [J].
COLDIRON, NB ;
YUKNA, RA ;
WEIR, J ;
CAUDILL, RF .
JOURNAL OF PERIODONTOLOGY, 1990, 61 (05) :293-299