A hairy root culture of melon produces aroma compounds

被引:10
作者
Matsuda, Y
Toyoda, H
Sawabe, A
Maeda, K
Shimizu, N
Fujita, N
Fujita, T
Nonomura, T
Ouchi, S
机构
[1] Kinki Univ, Inst Comprehens Agr Sci, Div Bioengn & Organ Chem, Nara 6318505, Japan
[2] Kinki Univ, Fac Agr, Lab Plant Pathol & Biotechnol, Nara 6318505, Japan
[3] Kinki Univ, Expt Farm, Wakayama 6430004, Japan
关键词
melon hairy root; T-DNA insertion; inactivation; Agrobacterium rhizogenes; aroma volatiles with fruity fragrance;
D O I
10.1021/jf9906580
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Musk melon is the favorite fruit with a high market value in Japan, and the fragrance is one of the major factors determining the fruit quality of melon. In this study, mutant melon hairy roots which had been induced by means of the T-DNA insertion mutagenesis were found to produce volatile compounds with the fruity fragrance of mature melon. The volatile compounds were extracted and identified by GLC-mass spectrometry. Some essential oils such as (Z)-3-hexenol, (E)-2-hexenal, 1-nonanol, and (Z)-6-nonenol were stably synthesized by these hairy roots despite the increased number of subcultures. The productivity of these compounds by the best hairy root line was shown to be considerably higher than naturally ripened melon fruits.
引用
收藏
页码:1417 / 1420
页数:4
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