The membrane-bound respiratory chain of Pseudomonas pseudoalcaligenes KF707 cells grown in the presence or absence of potassium tellurite

被引:44
作者
Di Tomaso, G [1 ]
Fedi, S [1 ]
Carnevali, M [1 ]
Manegatti, M [1 ]
Taddei, C [1 ]
Zannoni, D [1 ]
机构
[1] Univ Bologna, Dept Biol, I-40126 Bologna 1, Italy
来源
MICROBIOLOGY-SGM | 2002年 / 148卷
关键词
cytochromes; tellurite uptake; tellurium crystallites;
D O I
10.1099/00221287-148-6-1699
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The respiratory chain of Pseudomonas pseudoalcaligenes KF707 in membranes isolated from cells grown in the presence or absence of the toxic oxyanion tellurite (TeO32-) was examined. Aerobic growth in the absence of tellurite shows an NADH-dependent respiration which is 80% catalysed by the cytochrome (cyt) bc(1)-containing pathway leading to two terminal membrane-bound cyt c oxidases inhibited by different concentrations of KCN (IC50 0.2 and 1 muM). A third oxidase, catalysing the remaining 20% of the cyanide-resistant respiration and fully inhibited by 2-3 mM KCN, is also present; this latter pathway accounts for 60-70% of the total NADH-dependent respiration in membranes from cells grown in LB medium supplemented with potassium tellurite (35 mug ml(-1)). Two high-potential b-type haems (E-m,E-7 +395 and 318 mV) are redox centres of a membrane-bound cyt c oxiclase (possibly of the cbb(3) type) which shows a 50% decrease of its activity in parallel with a similar decrease of the c-type haem content (mostly soluble cyt c) in membranes from tellurite-grown cells; the latter type of cells specifically contain a cyt b type at +203 mV (pH 9-0) which is likely to be involved in cyanide-resistant respiration. Comparison of the growth curve of KF707 cells in parallel with tellurite uptake showed that intracellular accumulation of tellurium (Te-0) crystallites starts from the mid-exponential growth phase, whereas tellurite-induced changes of the respiratory chain are already evident during the early stages of growth. These data were interpreted as showing that reduction of tellurite to tellurium and tellurite-dependent modifications of the respiratory chain are unrelated processes in P. pseudoalcaligenes KF707.
引用
收藏
页码:1699 / 1708
页数:10
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