PUF60: A novel U2AF65-related splicing activity

被引：109

作者：

Page-McCaw, PS

Amonlirdviman, K

Sharp, PA

机构：

[1] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

RNA | 1999年 / 5卷 / 12期

关键词：

p54; PUF60; pump domain; pyrimidine tract; RRM; splicing factor; U2AF;

D O I：

10.1017/S1355838299991938

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a new pyrimidine-tract binding factor, PUF, that is required, together with U2AF, for efficient reconstitution of RNA splicing in vitro. The activity has been purified and consists of two proteins, PUF60 and the previously described splicing factor p54. p54 and PUF60 form a stable complex in vitro when cotranslated in a reaction mixture. PUP activity, in conjunction with U2AF, facilitates the association of U2 snRNP with the pre-mRNA, This reaction is dependent upon the presence of the large subunit of U2AF, U2AF65, but not the small subunit U2AF35, PUF60 is homologous to both U2AF65 and the yeast splicing factor Mud2p, The C-terminal domain of PUF60, the PUMP domain, is distantly related to the RNA-recognition motif domain, and is probably important in protein-protein interactions.

引用

收藏

页码：1548 / 1560

页数：13

相关论文

共 48 条

[1] THE YEAST MUD2 PROTEIN - AN INTERACTION WITH PRP11 DEFINES A BRIDGE BETWEEN COMMITMENT COMPLEXES AND U2 SNRNP ADDITION [J].

ABOVICH, N ;

LIAO, XLC ;

ROSBASH, M .

GENES & DEVELOPMENT, 1994, 8 (07) :843-854

[2] Novel proteins that interact with the COOH-terminal cytosolic routing determinants of an integral membrane peptide-processing enzyme [J].

Alam, MR ;

Caldwell, BD ;

Johnson, RC ;

Darlington, DN ;

Mains, RE ;

Eipper, BA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (45) :28636-28640

[3]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[4] TARGETED SNRNP DEPLETION REVEALS AN ADDITIONAL ROLE FOR MAMMALIAN U1 SNRNP IN SPLICEOSOME ASSEMBLY [J].

BARABINO, SML ;

BLENCOWE, BJ ;

RYDER, U ;

SPROAT, BS ;

LAMOND, AI .

CELL, 1990, 63 (02) :293-302

[5] The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC [J].

Berglund, JA ;

Chua, K ;

Abovich, N ;

Reed, R ;

Rosbash, M .

CELL, 1997, 89 (05) :781-787

[6] A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition [J].

Berglund, JA ;

Abovich, N ;

Rosbash, M .

GENES & DEVELOPMENT, 1998, 12 (06) :858-867

[7] ANALYSIS OF THE RNA-RECOGNITION MOTIF AND RS AND RGG DOMAINS - CONSERVATION IN METAZOAN PRE-MESSENGER-RNA SPLICING FACTORS [J].

BIRNEY, E ;

KUMAR, S ;

KRAINER, AR .

NUCLEIC ACIDS RESEARCH, 1993, 21 (25) :5803-5816

[8] PRIMARY STRUCTURE OF A HUMAN ARGININE-RICH NUCLEAR-PROTEIN THAT COLOCALIZES WITH SPLICEOSOME COMPONENTS [J].

CHAUDHARY, N ;

MCMAHON, C ;

BLOBEL, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :8189-8193

[9] SPECIFIC PROTEIN-PROTEIN INTERACTIONS BETWEEN THE ESSENTIAL MAMMALIAN SPLICEOSOME-ASSOCIATED PROTEINS SAP-61 AND SAP-114 [J].

CHIARA, MD ;

CHAMPIONARNAUD, P ;

BUVOLI, M ;

NADALGINARD, B ;

REED, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6403-6407

[10]

Creighton T.E., 1997, PROTEINS STRUCTURES, VSecond

← 1 2 3 4 5 →