Starting a new genetic system:: Lessons from Bacteroides

被引：43

作者：

Salyers, AA ^{[1
]}

Bonheyo, G ^{[1
]}

Shoemaker, NB ^{[1
]}

机构：

[1] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

来源：

METHODS | 2000年 / 20卷 / 01期

关键词：

D O I：

10.1006/meth.1999.0903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite the fact that genetic manipulation is an essential adjunct of studies of microbial physiology, many important bacterial species are still not accessible to genetic manipulation. In many cases, the people who know the organism best and have studied its physiology extensively have little experience with genetic techniques and are uncertain how to set up a new system. In this article, we describe what we have learned in the process of developing a genetic system for Bacteroides spp. and helping to develop genetic tools for two related species of bacteria: Porphromonas gingivalis and Prevotella ruminicola. We discuss the relative usefulness of different types of genetic tools and make some suggestions about how to construct them. (C) 2000 Academic Press.

引用

页码：35 / 46

页数：12

共 26 条

[1] Construction and characterization of a Bacteroides thetaiotaomicron recA mutant: Transfer of Bacteroides integrated conjugative elements is RecA independent [J].