In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis

被引：16

作者：

Koga, Y ^{[1
]}

Kobayashi, K ^{[1
]}

Yang, JH ^{[1
]}

Nakano, H ^{[1
]}

Yamane, T ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Biol & Agr Sci, Lab Mol Biotechnol, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2002年 / 94卷 / 01期

基金：

日本学术振兴会;

关键词：

Burkholderia cepacia; in vitro protein synthesis; lipase; protein engineering; protein library construction;

D O I：

10.1263/jbb.94.84

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity.

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收藏

页码：84 / 86

页数：3

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