1.7 Å X-ray structure of space-grown collagenase crystals

被引：11

作者：

Broutin-L'Hermite, I ^{[1
]}

Ries-Kautt, M ^{[1
]}

Ducruix, A ^{[1
]}

机构：

[1] CNRS, Lab Enzymol & Biochim Struct, F-91198 Gif Sur Yvette, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444999016789

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Collagenases, divided into metallocollagenases and serine collagenases, are the only proteases that cleave the triple helix of collagen under physiological conditions. In the present work, the serine protease collagenase purified from Hypoderma lineatum larvae is studied. From crystals grown in the international Microgravity Laboratory (IML2), a data set was collected at 1.7 Angstrom using synchrotron radiation. Although the resolution is not very different, the signal-to-noise ratio and the quality of the electron density are much improved. Alternate conformations were revealed for several residues, in particular Tyr99, suggesting a gate mechanism of recognition.

引用

页码：376 / 378

页数：3

共 22 条

[1]

ANZHI W, 1998, FEBS LETT, V234, P367

[2] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[3] 1.8-angstrom crystal structure of the catalytic domain of human neutrophil collagenase (matrix metalloproteinase-8) complexed with a peptidomimetic hydroxamate prime-side inhibitor with a distinct selectivity profile [J].

Betz, M ;

Huxley, P ;

Davies, SJ ;

Mushtaq, Y ;

Pieper, M ;

Tschesche, H ;

Bode, W ;

GomisRuth, FX .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 247 (01) :356-363

[4] EXPERIMENT EQUIPMENT FOR PROTEIN CRYSTALLIZATION IN MU-G FACILITIES [J].

BOSCH, R ;

LAUTENSCHLAGER, P ;

POTTHAST, L ;

STAPELMANN, J .

JOURNAL OF CRYSTAL GROWTH, 1992, 122 (1-4) :310-316

[5] CHARACTERIZATION OF A COLLAGENOLYTIC ENZYME FROM LARVAE OF HYPODERMA-LINEATUM (INSECTA DIPTERA, OESTRIFORM) [J].

BOULARD, C ;

GARRONE, R .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1978, 59 (03) :251-255

[6] Structure of malonic acid-based inhibitors bound to human neutrophil collagenase. A new binding mode explains apparently anomalous data [J].

Brandstetter, H ;

Engh, RA ;

Von Roedern, EG ;

Moroder, L ;

Huber, R ;

Bode, W ;

Grams, F .

PROTEIN SCIENCE, 1998, 7 (06) :1303-1309

[7] 1.8 angstrom structure of Hypoderma lineatum collagenase: A member of the serine proteinase family [J].

Broutin, I ;

Arnoux, B ;

Riche, C ;

Lecroisey, A ;

Keil, B ;

Pascard, C ;

Ducruix, A .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1996, 52 :380-392

[8] Crystallographic analyses of lysozyme and collagenase microgravity grown crystals versus ground controls [J].

Broutin, I ;

RiesKautt, M ;

Ducruix, A .

JOURNAL OF CRYSTAL GROWTH, 1997, 181 (1-2) :97-108

[9] FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES [J].

BRUNGER, AT .

NATURE, 1992, 355 (6359) :472-475

[10]

BRUNGER AT, 1992, XPLOR VERSION 3 851

← 1 2 3 →