Anti-angiogenic effects of homocysteine on cultured endothelial cells

被引：35

作者：

Rodríguez-Nieto, S ^{[1
]}

Chavarría, T ^{[1
]}

Martínez-Poveda, B ^{[1
]}

Sánchez-Jiménez, F ^{[1
]}

Quesada, AR ^{[1
]}

Medina, MA ^{[1
]}

机构：

[1] Univ Malaga, Fac Sci, Dept Mol Biol & Biochem, E-29071 Malaga, Spain

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 01期

关键词：

homocysteine; angiogenesis; matrix metalloproteinase-2; urokinase;

D O I：

10.1016/S0006-291X(02)00232-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High levels of homocysteine induce a sustained injury on arterial endothelial cells which accelerates the development of thrombosis and atherosclerosis. Some of the described effects of homocysteine on endothelial cells are features shared with an antiangiogenic response. Therefore, we studied the effects of homocysteine on key steps of angiogenesis using bovine aorta endothelial cells as a model. Homocysteine decreased proliferation and induced differentiation. Furthermore. 5mM homocysteine produced strong inhibitions of matrix metalloproteinase-2 and urokinase. two proteolytic activities that play a key role in extracellular matrix re-modeling, and decreased migration and invasion. other two key steps of angiogenesis, This study demonstrates that homocysteine can inhibit several steps of the angiogenic process. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：497 / 500

页数：4

共 24 条

[1] Efficiency of homocysteine plus copper in inducing apoptosis is inversely proportional to γ-glutamyl transpeptidase activity [J].

Bessede, G ;

Miguet, C ;

Gambert, P ;

Neel, D ;

Lizard, G .

FASEB JOURNAL, 2001, 15 (11) :1927-1940

[2] Direct interaction between endothelial nitric-oxide synthase and dynamin-2 - Implications for nitric-oxide synthase function [J].

Cao, S ;

Yao, J ;

McCabe, TJ ;

Yao, Q ;

Katusic, ZS ;

Sessa, WC ;

Shah, V .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (17) :14249-14256

[3]

CAVALLERO U, 2001, J CELL BIOCHEM, V82, P19

[4]

DAngelo A, 1997, BLOOD, V90, P1

[5] Modulation of the proteolytic balance plasminogen activator plasminogen activator inhibitor by enhanced N-myc oncogene expression or application of genistein [J].

de Veas, RG ;

Schweigerer, L ;

Medina, MA .

EUROPEAN JOURNAL OF CANCER, 1998, 34 (11) :1736-1740

[6] Homocysteine-induced decrease in endothelin-1 production is initiated at the extracellular level and involves oxidative products [J].

Drunat, S ;

Moatti, N ;

Paul, JL ;

Cogny, A ;

Benoit, MO ;

Demuth, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (20) :5287-5294

[7] Halofuginone: a potent inhibitor of critical steps in angiogenesis progression [J].

Elkin, M ;

Miao, HQ ;

Nagler, A ;

Aingorn, E ;

Reich, R ;

Hemo, I ;

Dou, HL ;

Pines, M ;

Vlodavsky, I .

FASEB JOURNAL, 2000, 14 (15) :2477-2485

[8] A comparative study of the effects of genistein and 2-methoxyestradiol on the proteolytic balance and tumour cell proliferation [J].

Fajardo, I ;

Quesada, AR ;

de Castro, IN ;

Sánchez-Jiménez, F ;

Medina, MA .

BRITISH JOURNAL OF CANCER, 1999, 80 (1-2) :17-24

[9] ANGIOGENESIS IN CANCER, VASCULAR, RHEUMATOID AND OTHER DISEASE [J].

FOLKMAN, J .

NATURE MEDICINE, 1995, 1 (01) :27-31

[10] GENISTEIN, A DIETARY-DERIVED INHIBITOR OF INVITRO ANGIOGENESIS [J].

FOTSIS, T ;

PEPPER, M ;

ADLERCREUTZ, H ;

FLEISCHMANN, G ;

HASE, T ;

MONTESANO, R ;

SCHWEIGERER, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :2690-2694

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