The nucleocapsid-binding spike subunit E2 of Semliki Forest virus requires complex formation with the E1 subunit for activity

被引:18
作者
Barth, BU
Garoff, H
机构
关键词
D O I
10.1128/JVI.71.10.7857-7865.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Alphaviruses, such as Semliki Forest virus (SFV), mature by budding at the plasma membrane (Phl) of infected cells and enter uninfected ones by a membrane fusion process in the endosomes, Both processes are directed by the p62/E2-E1 membrane protein heterodimer of the virus. The p62 protein, or its mature form E2, provides a cytoplasmic protein domain for interaction with the nucleocapsid (NC) of the virus, and the Fl protein functions as a membrane fusogen. We have previously shown that the p62/E2 protein of SFV controls the membrane fusion activity of El through its complex formation with the latter (A, Salminen, J. hi. Wahlberg, M. Lobigs, P. Liljestrom, and H. Garoff, J. Cell Biol, 116:339-357, 1992). In the present work, we show that the El protein controls the NC-binding activity of p62/E2. We have studied E1 expression-deficient SFV variants and shown that although the p62/EZ proteins can be transported to the PR I they cannot establish stable NC associations.
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页码:7857 / 7865
页数:9
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