Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease

被引：125

作者：

McClements, WL ^{[1
]}

Armstrong, ME ^{[1
]}

Keys, RD ^{[1
]}

Liu, MA ^{[1
]}

机构：

[1] MERCK RES LABS, DEPT VIRUS & CELL BIOL, West Point, PA 19486 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 21期

关键词：

herpes simplex virus; nucleic acid; glycoprotein;

D O I：

10.1073/pnas.93.21.11414

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA vaccines expressing herpes simplex virus type 2 (HSV-2) full-length glycoprotein D (gD), or a truncated form of HSV-2 glycoprotein B (gB) were evaluated for protective efficacy in two experimental models of HSV-2 infection. Intramuscular (i.m.) injection of mice showed that each construction induced neutralizing serum antibodies and protected the mice from lethal HSV-2 infection. Dose-titration studies showed that low doses (less than or equal to 1 mu g) of either DNA construction induced protective immunity, and that a single immunization with the go construction was effective. The two DNAs were then tested in a low-dosage combination in guinea pigs. Immune sera from DNA-injected animals had antibodies to both go and gB, and virus neutralizing activity. When challenged by vaginal infection with HSV-2, the DNA-immunized animals were significantly protected from primary genital disease.

引用

页码：11414 / 11420

页数：7

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