The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion

被引：32

作者：

Amer, AO

Valvano, MA ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Microbiol & Immunol, London, ON N6A 5C1, Canada

[2] Univ Western Ontario, Dept Med, London, ON N6A 5C1, Canada

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 02期

关键词：

D O I：

10.1128/JB.182.2.498-503.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.

引用

页码：498 / 503

页数：6

共 39 条

[1] ROLE OF THE RFE GENE IN THE BIOSYNTHESIS OF THE ESCHERICHIA-COLI O7-SPECIFIC LIPOPOLYSACCHARIDE AND OTHER O-SPECIFIC POLYSACCHARIDES CONTAINING N-ACETYLGLUCOSAMINE
ALEXANDER, DC
VALVANO, MA
[J]. JOURNAL OF BACTERIOLOGY, 1994, 176 (22) : 7079 - 7084
[2] PBLUESCRIPT-II - GENE-MAPPING VECTORS
ALTINGMEES, MA
SHORT, JM
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (22) : 9494 - 9494
[3] AMER A, UNPUB
[4] BARR K, 1987, J BIOL CHEM, V262, P7142
[5] ROLE OF RFE AND RFBF IN THE INITIATION OF BIOSYNTHESIS OF D-GALACTAN-I, THE LIPOPOLYSACCHARIDE O-ANTIGEN FROM KLEBSIELLA-PNEUMONIAE SEROTYPE-O1
CLARKE, BR
BRONNER, D
KEENLEYSIDE, WJ
SEVERN, WB
RICHARDS, JC
WHITFIELD, C
[J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (19) : 5411 - 5418
[6] Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method
Cserzo, M
Wallin, E
Simon, I
vonHeijne, G
Elofsson, A
[J]. PROTEIN ENGINEERING, 1997, 10 (06): : 673 - 676
[7] DIRECTIONALITY IN PROTEIN TRANSLOCATION ACROSS MEMBRANES - THE N-TAIL PHENOMENON
DALBEY, RE
KUHN, A
VONHEIJNE, G
[J]. TRENDS IN CELL BIOLOGY, 1995, 5 (10) : 380 - 383
[8] DALNOGARE AR, 1988, GLYCOBIOLOGY, V8, P625
[9] Hamster UDP-N-acetylglucosamine:dolichol-P N-acetylglucosamine-1-P transferase has multiple transmembrane spans and a critical cytosolic loop
Dan, N
Middleton, RB
Lehrman, MA
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (48) : 30717 - 30724
[10] Oligomerization of hamster UDP-GlcNAc:Dolichol-P GlcNAc-1-P transferase, an enzyme with multiple transmembrane spans
Dan, N
Lehrman, MA
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (22) : 14214 - 14219

← 1 2 3 4 →