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In vivo bioluminescence imaging of the murine pathogen Citrobacter rodentium

被引：94
作者：
Wiles, Siouxsie
Pickard, Karen M.
Peng, Katian
MacDonald, Thomas T.
Frankel, Gad
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Div Cell & Mol Biol, Fac Nat Sci, London SW7 2AZ, England
[2] Univ Southampton, Sch Med, Div Infect Inflammat & Repair, Southampton SO16 6YD, Hants, England
[3] Barts & London Sch Med & Dent, Inst Cell & Mol Sci, London E1 2AT, England
来源：
INFECTION AND IMMUNITY | 2006年 / 74卷 / 09期
基金：
英国惠康基金;
关键词：
D O I：
10.1128/IAI.00848-06
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Citrobacter rodentium is a natural mouse pathogen related to enteropathogenic and enterohemorrhagic Escherichia coli. We have previously utilized bioluminescence imaging (BLI) to determine the in vivo colonization dynamics of C rodentium. However, due to the oxygen requirement of the bioluminescence system and the colonic localization of C. rodentium, in vivo localization studies were performed using harvested organs. Here, we report the detection of bioluminescent C rodentium and commensal E. coli during colonization of the gastrointestinal tract in intact living animals. Bioluminescence was dependent on intact blood circulation, suggesting that the colonic environment is not anaerobic but nanaerobic. In addition, BLI revealed that C. rodentium colonizes the rectum, a site previously unreported for this pathogen.
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页码：5391 / 5396
页数：6
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