Crystallization and preliminary X-ray crystallographic studies of rice α-galactosidase

被引：5

作者：

Fujimoto, Z

Kobayashi, O

Kaneko, S

Momma, M

Kobayashi, H

Mizuno, H ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Dept Biochem, Tsukuba, Ibaraki 3058602, Japan

[2] Natl Food Res Inst, Biol Funct Div, Tsukuba, Ibaraki 3058642, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444902010168

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Galactosidases catalyze the hydrolysis of galactooligosaccharides and galactopolysaccharides to alpha-galactose residues and are widely distributed in microorganisms, plants and animals. alpha-Galactosidase from rice (Oryza sativa L. ssp. japonica) was crystallized by the hanging-drop vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 63.1, b = 71.3, c = 85.6 Angstrom, and diffract beyond 1.9 Angstrom resolution.

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页码：1374 / 1375

页数：2

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