Automated PCR/sequence template purification

被引：16

作者：

Clarke, SC ^{[1
]}

Diggle, MA ^{[1
]}

机构：

[1] Stobhill Gen Hosp, Dept Microbiol, Scottish Meningococcus & Pneumococcus Reference L, Glasgow G21 3UW, Lanark, Scotland

来源：

MOLECULAR BIOTECHNOLOGY | 2002年 / 21卷 / 03期

关键词：

DNA sequencing; polymerase chain reaction; automation; purification;

D O I：

10.1385/MB:21:3:221

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.

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收藏

页码：221 / 224

页数：4

相关论文

共 14 条

[1]

Andersson B, 1996, BIOTECHNIQUES, V20, P1022

[2] Isolation of genomic DNA from blood using a novel filter-based DNA purification technology [J].

Baker, MP ;

Mitchell, A ;

Bridge, C ;

Martin, S ;

Holder, F ;

Pathirana, ND ;

Jarvis, A ;

Butt, N .

BIOTECHNIQUES, 2001, 31 (01) :142-145

[3] Molecular techniques: divide or share [J].

Boxer, M .

JOURNAL OF CLINICAL PATHOLOGY, 2000, 53 (01) :19-21

[4] Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis [J].

Clarke, SC ;

Diggle, MA ;

Edwards, GFS .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) :3066-3071

[5] Introduction of an automated service for the laboratory confirmation of meningococcal disease in Scotland [J].

Clarke, SC ;

Diggle, MA ;

Reid, JA ;

Thom, L ;

Edwards, GFS .

JOURNAL OF CLINICAL PATHOLOGY, 2001, 54 (07) :556-557

[6] Multilocus sequence typing [J].

Enright, MC ;

Spratt, BG .

TRENDS IN MICROBIOLOGY, 1999, 7 (12) :482-487

[7] A multilocus sequence typing scheme for Streptococcus pneumoniae:: identification of clones associated with serious invasive disease [J].

Enright, MC ;

Spratt, BG .

MICROBIOLOGY-UK, 1998, 144 :3049-3060

[8] SIZE FRACTIONATION OF DOUBLE-STRANDED DNA BY PRECIPITATION WITH POLYETHYLENE-GLYCOL [J].

LIS, JT ;

SCHLEIF, R .

NUCLEIC ACIDS RESEARCH, 1975, 2 (03) :383-389

[9] Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms [J].

Maiden, MCJ ;

Bygraves, JA ;

Feil, E ;

Morelli, G ;

Russell, JE ;

Urwin, R ;

Zhang, Q ;

Zhou, JJ ;

Zurth, K ;

Caugant, DA ;

Feavers, IM ;

Achtman, M ;

Spratt, BG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (06) :3140-3145

[10] Principles and applications of methods for DNA-beased typing of microbial organisms [J].

Olive, DM ;

Bean, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) :1661-1669