Biotechnological aspects of chitinolytic enzymes: a review

被引：501

作者：

Dahiya, Neetu

Tewari, Rupinder

Hoondal, Gurinder Singh

机构：

[1] Natl Inst Immunol, Genes & Prot Lab, New Delhi 110067, India

[2] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India

[3] Panjab Univ, Dept Microbiol, Chandigarh 160014, India

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 71卷 / 06期

关键词：

D O I：

10.1007/s00253-005-0183-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Chitin and chitinases (EC 3.2.1.14) have an immense potential. Chitinolytic enzymes have wide-ranging applications such as preparation of pharmaceutically important chitooligosacchandes and N-acetyl D-glucosamine, preparation of single-cell protein, isolation of protoplasts from fungi and yeast, control of pathogenic fungi, treatment of chitinous waste, and control of malaria transmission. In this review, we discuss the occurrence and structure of chitin, the types and sources of chitinases, their mode of action, chitinase production, as well as molecular cloning and protein engineering of chitinases and their biotechnological applications.

引用

页码：773 / 782

页数：10

共 89 条

[1]

CLONING AND SEQUENCING OF CHIC GENE OF BACILLUS-CIRCULANS WL-12 AND RELATIONSHIP OF ITS PRODUCT TO SOME OTHER CHITINASES AND CHITINASE-LIKE PROTEINS [J].