Implication of mitochondria-derived ROS and cardiolipin peroxiclation in N-(4-hydroxyphenyl)retinamide-induced apoptosis

被引:83
作者
Asumendi, A [1 ]
Morales, MC [1 ]
Alvarez, A [1 ]
Aréchaga, J [1 ]
Pérez-Yarza, H [1 ]
机构
[1] Univ Basque Country, Sch Med & Dent, Dept Cell Biol & Histol, Leioa 48940, Bizkaia, Spain
关键词
N-(4-hydroxyphenyl)retinamide; apoptosis; reactive oxygen species; mitochondria; cardiolipin peroxidation; cytochrome c;
D O I
10.1038/sj.bjc.6600356
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have studied the effect of N-(4-hydroxyphenyl)retinamide on either malignant human leukaemia cells or normal cells and investigated its mechanism of action. We demonstrate that 4HPR induces reactive oxygen species increase on mitochondria at a target between mitochondrial respiratory chain complex I and II. Such oxidative stress causes cardiolipin peroxidation which in turn allows cytochrome c release to cytosol, caspase-3 activation and therefore apoptotic consumption. Moreover, this apoptotic pathway seems to be bcl-2/bax independent and count only on malignant cells but not normal nor activated lymphocytes. (C) 2002 Cancer Research UK.
引用
收藏
页码:1951 / 1956
页数:6
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