Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification

被引:6
作者
Blaga, R. [1 ]
Fu, BaoQuan [2 ]
Le Rhun, D. [3 ]
Le Naour, E. [3 ]
Heckman, A. [3 ]
Zocevic, A. [3 ]
Liu, MingYuan [4 ]
Boireau, P. [3 ]
机构
[1] Univ Agr Sci & Vet Med, Cluj Napoca 400372, Romania
[2] Chinese Acad Agr Sci, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China
[3] UPVM, JRU, INRA, BIPAR,AFSSA,ENVA, F-94703 Maisons Alfort, France
[4] Jilin Univ, Key Lab Zoonoses, Minist Educ, Inst Zoonoses, Changchun 130062, Peoples R China
关键词
POLYMERASE-CHAIN-REACTION; FRAGMENT-LENGTH-POLYMORPHISM; PARASITIC NEMATODES; IDENTIFICATION; SPIRALIS; DNA; PSEUDOSPIRALIS; BRITOVI; POPULATIONS; SYSTEMATICS;
D O I
10.1017/S0022149X09359830
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Until now, four species of the Trichinella genus have been identified in Europe: Trichinella spiralis, T. nativa, T britovi and T pseudospiralis. The aim of this work was to establish a sound polymerase chain reaction (PCR)-based method to differentiate these four species using mitochondrial rDNA as a reliable genetic marker and to evaluate the sensitivity of this method. Full-length DNA sequences coding for the small and large mitochondrial rRNA (mt-rrnS and mt-rrnL) of the four species are described. A multiplex PCR was designed and successfully tested on 24 European isolates. As few as one larva, or 100 pg of genomic DNA was detected, providing equivalent sensitivity to previously described PCR methods. The PCR-based method of mitochondrial rDNA amplification was thereby established as a sensitive and reproductive diagnostic method for the four European Trichinella species.
引用
收藏
页码:121 / 128
页数:8
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