Reduced host resistance and Th1 response to Cryptococcus neoformans in interleukin-18 deficient mice

被引:39
作者
Kawakami, K
Koguchi, Y
Qureshi, MH
Kinjo, Y
Yara, S
Miyazato, A
Kurimoto, M
Takeda, K
Akira, S
Saito, A
机构
[1] Univ Ryukyus, Fac Med, Dept Internal Med 1, Okinawa 9030215, Japan
[2] Hayashibara Biochem Labs Inc, Fujisaki Inst, Okayama, Japan
[3] Osaka Univ, Microbial Dis Res Inst, Dept Host Def, Osaka, Japan
关键词
IL-18; IL-12; DTH; Th1; response; Cryptococcus neoformans;
D O I
10.1111/j.1574-6968.2000.tb09092.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using interleukin (IL)-18 deficient (IL-18(-/-)) mice, we examined the role of IL-18 in the host resistance and Th1 response against infection with Cryptococcus neoformans. Fungal clearance in the lung was reduced in IL-18(-/-) mice, although there was no significant change in the level of dissemination to the brain. The DTH response, as determined by footpad swelling, was also diminished in IL-18(-/-) mice compared to control wild-type (WT) mice. The levels of IL-12 and interferon (IFN)-gamma in the sera were significantly lower in IL-18(-/-) mice than in WT mice. Spleen cells from infected WT mice produced a high level of IFN-gamma upon stimulation with the microbe, while only a low level of IFN-gamma production was detected in spleen cells from infected IL-18(-/-) mice; Administration of IL-18 almost completely restored the reduced response in IL-18(-/-) mice, while IL-12 showed a marginal effect. These results demonstrated the important role of IL-18 in the resistance and Th1 response of mice to C. neoformans by potentiating the production of IFN-gamma. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:121 / 126
页数:6
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