A New Role of Anandamide in Human Sperm: Focus on Metabolism

被引:38
作者
Aquila, Saveria [1 ,2 ]
Guido, Carmela [1 ,2 ]
Laezza, Chiara [3 ]
Santoro, Antonietta [4 ]
Pezzi, Vincenzo [1 ,5 ]
Panza, Salvatore [1 ,2 ]
Ando, Sebastiano [1 ,2 ,5 ]
Bifulco, Maurizio [4 ]
机构
[1] Univ Calabria, Dept Pharmacobiol, I-87036 Cosenza, Italy
[2] Univ Calabria, Ctr Sanit, I-87036 Cosenza, Italy
[3] CNR, Inst Endocrinol & Expt Oncol, I-80125 Naples, Italy
[4] Univ Salerno, Dept Pharmaceut Sci, I-84100 Salerno, Italy
[5] Univ Calabria, Fac Pharm, I-87036 Cosenza, Italy
关键词
PROTEIN-TYROSINE PHOSPHORYLATION; PENTOSE-PHOSPHATE PATHWAY; CANNABINOID RECEPTOR; ENDOCANNABINOID SYSTEM; MOUSE SPERMATOZOA; ACROSOME REACTION; CAPACITATION; ETHANOLAMIDES; STIMULATION; EXPRESSION;
D O I
10.1002/jcp.21837
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The endocannabinoid system and the presence of CBI receptor (CBI-R) target of the anandamide were identified in human sperm, however the anandamide action in this context needs to be further elucidated. At this purpose we analyzed the effects of anandamide on human sperm capacitation and motility. Afterwards, we focused on lipid and glucose sperm metabolism and also investigated the interrelationship between anandamide and insulin secretion by sperm. By intracellular free Ca2+ content assay and proteins tyrosine phosphorylation, we evidenced that anandamide did not induce capacitation process and a negative effect was obtained on sperm motility. The blockage of CBI-R by the specific antagonist SR141716 increased both capacitation and sperm motility suggesting an involvement of the CBI-R in the acquisition of sperm fertilizing activity. The evaluation of the triglycerides content, lipase and acyl-CoA dehydrogenase activities, suggest that anandamide exerts a lipogenetic effect on human sperm lipid metabolism. Concerning the glucose metabolism, anandamide increases GSK3 phosphorylation indicating that it is involved in the accumulation of energy substrates. G6PDH activity was not affected by anandamide. Interestingly, AEA is involved in insulin secretion by sperm. As insulin had been demonstrated to be an autocrine factor that triggers capacitation, the endocannabinoid might be inserted in the signaling cascade that induces this process. Altogether these findings highlight a pivotal involvement of the CBI-R in the control of sperm energy homeostasis and propose a new site of action for endocannabinoids in the control of energy metabolism. J. Cell. Physiol. 221: 147-153, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:147 / 153
页数:7
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