A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease

被引：47

作者：

Don, Emily K. ^{[1
]}

Formella, Isabel ^{[1
]}

Badrock, Andrew P. ^{[1
]}

Hall, Thomas E. ^{[2
]}

Morsch, Marco ^{[1
]}

Hortle, Elinor ^{[1
]}

Hogan, Alison ^{[1
]}

Chow, Sharron ^{[1
]}

Gwee, Serene S. L. ^{[1
]}

Stoddart, Jack J. ^{[1
]}

Nicholson, Garth ^{[1
,3
]}

Chung, Roger ^{[1
]}

Cole, Nicholas J. ^{[1
]}

机构：

[1] Macquarie Univ, Dept Biomed Sci, Fac Med & Hlth Sci, Ground Floor,2 Technol Pl,F10A Bldg, Sydney, NSW 2109, Australia

[2] Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia

[3] Univ Sydney, ANZAC Res Inst, Concord Hosp, Sydney, NSW, Australia

来源：

ZEBRAFISH | 2017年 / 14卷 / 01期

基金：

英国医学研究理事会;

关键词：

transgenesis; neurodegenerative disease; Tol2; gateway; disease model; ZEBRAFISH; TRANSGENESIS; IDENTIFICATION; EXPRESSION; ENHANCER; ELEMENT;

D O I：

10.1089/zeb.2016.1321

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.

引用

页码：69 / 72

页数：4

共 13 条

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Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish [J].