Metabolic fingerprints in testicular biopsies from type 1 diabetic patients

被引：22

作者：

Alves, Marco G. ^{[1
]}

Martins, Ana D. ^{[1
,5
,6
]}

Moreira, Paula I. ^{[2
,3
]}

Carvalho, Rui A. ^{[3
,4
]}

Sousa, Mario ^{[5
,6
,7
]}

Barros, Alberto ^{[7
,8
]}

Silva, Joaquina ^{[7
]}

Pinto, Soraia ^{[9
]}

Simoes, Teresinha ^{[9
]}

Oliveira, Pedro Fontes ^{[1
,5
,6
]}

机构：

[1] Univ Beira Interior, Hlth Sci Res Ctr, CICS, P-6201506 Covilha, Portugal

[2] Univ Coimbra, Fac Med, Physiol Lab, P-3000548 Coimbra, Portugal

[3] Univ Coimbra, Ctr Neurosci & Cell Biol, CNC, P-3004517 Coimbra, Portugal

[4] Univ Coimbra, Fac Sci & Technol FCTUC, Dept Life Sci, P-3004517 Coimbra, Portugal

[5] Univ Porto, Inst Biomed Sci Abel Salazar ICBAS, Cell Biol Lab, Dept Microscopy, P-4050313 Oporto, Portugal

[6] Univ Porto, Multidisciplinary Unit Biomed Res UMIB, P-4050313 Oporto, Portugal

[7] Ctr Reprod Genet Prof Alberto Barros, P-4100009 Oporto, Portugal

[8] Univ Porto, Fac Med, Dept Genet, P-4200319 Oporto, Portugal

[9] CHLC, Ctr Reprod Med Matern Dr Alfredo da Costa, P-1069089 Lisbon, Portugal

来源：

CELL AND TISSUE RESEARCH | 2015年 / 362卷 / 02期

关键词：

Testes; Diabetes; Metabolism; Spermatogenesis; Lactate; RAT SERTOLI-CELLS; MALE REPRODUCTIVE FUNCTION; HIGH-ENERGY DIETS; GLUCOSE-METABOLISM; IN-VITRO; INSULIN DEPRIVATION; GLYCOLYTIC PROFILE; RIBONUCLEIC-ACID; OXIDATIVE STRESS; MALE-FERTILITY;

D O I：

10.1007/s00441-015-2217-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Diabetes mellitus (DM) is a metabolic disease that has grown to pandemic proportions. Recent reports have highlighted the effect of DM on male reproductive function. Here, we hypothesize that testicular metabolism is altered in type 1 diabetic (T1D) men seeking fertility treatment. We propose to determine some metabolic fingerprints in testicular biopsies of diabetic patients. For that, testicular tissue from five normal and five type 1 diabetic men was analyzed by high-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. mRNA and protein expression of glucose transporters and glycolysis-related enzymes were also evaluated. Our results show that testes from diabetic men presented decreased levels of lactate, alanine, citrate and creatine. The mRNA levels of glucose transporter 1 (GLUT1) and phosphofructokinase 1 (PFK1) were decreased in testes from diabetic men but only GLUT3 presented decreased mRNA and protein levels. Lactate dehydrogenase (LDH) and glutamate pyruvate transaminase (GPT) protein levels were also found to be decreased in testes from diabetic men. Overall, our results show that T1D alters glycolysis-related transporters and enzymes, compromising lactate content in the testes. Moreover, testicular creatine content was severely depressed in T1D men. Since lactate and creatine are essential for germ cells development and support, the data discussed here open new insights into the molecular mechanism by which DM promotes subfertility/infertility in human males.

引用

页码：431 / 440

页数：10

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