Preparative Isolation of Alisol B and Alisol B 23-Acetate from Alismatis Rhizoma by Centrifugal Partition Chromatography Coupled with ELSD

被引：5

作者：

Yoon, Kee Dong ^{[1
]}

Chin, Young-Won ^{[1
]}

Ahn, Mi-Jeong ^{[2
]}

Kim, Jinwoong ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Seoul, South Korea

[2] Seoul Natl Univ, Pharmaceut Sci Res Inst, Seoul, South Korea

来源：

CHROMATOGRAPHIA | 2009年 / 69卷 / 7-8期

关键词：

Liquid-liquid chromatography; Centrifugal partition chromatography; Evaporative light scattering detection; Alisma orientale; Alisol B and alisol B 23-acetate; COUNTER-CURRENT CHROMATOGRAPHY; TERPENE COMPONENTS; METHANOL EXTRACT; DRIED RHIZOME; PURIFICATION; TRITERPENE;

D O I：

10.1365/s10337-008-0943-5

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Centrifugal partition chromatography coupled with evaporative light scattering detection was applied to separate and purify alisol B and alisol B 23-acetate from a chloroform soluble extract of Alisma orientale. The chloroform soluble fraction (870 mg) of A. orientale was separated utilizing a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10:2:10:7, v/v) to give alisol B (37.5 mg) and alisol B 23-acetate (205.3 mg). The purities of the two triterpenoids were determined to be over 98% by LC-ELSD and their structures were elucidated by ESI-MS, H-1 NMR and C-13 NMR spectroscopy.

引用

页码：791 / 793

页数：3

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