High-level resistance to ceftazidime conferred by a novel enzyme, CTX-M-32, derived from CTX-M-1 through a single Asp240-Gly substitution

被引：78

作者：

Cartelle, M ^{[1
]}

Tomas, MD ^{[1
]}

Molina, F ^{[1
]}

Moure, R ^{[1
]}

Villanueva, R ^{[1
]}

Bou, G ^{[1
]}

机构：

[1] Complejo Hosp Univ Juan Canalejo, Microbiol Serv, La Coruna 15006, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 06期

关键词：

D O I：

10.1128/AAC.48.6.2308-2313.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (bla(CTX-M-32)) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed mutagenesis we demonstrated that this replacement is a key event in ceftazidime hydrolysis.

引用

页码：2308 / 2313

页数：6

共 26 条

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