Presentation and detection of azide functionality in bacterial cell surface proteins

被引：261

作者：

Link, AJ ^{[1
]}

Vink, MKS ^{[1
]}

Tirrell, DA ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 34期

关键词：

D O I：

10.1021/ja047629c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An improved protocol for copper-catalyzed triazole formation on the bacterial cell surface is described. Addition of highly pure CuBr to cells treated with azidohomoalanine (2) leads to ca. 10-fold more extensive cell surface labeling than previously observed. This highly active catalyst allows detection of the methionine analogues azidoalanine (1), azidonorvaline (3), and azidonorleucine (4) in cell surface proteins. Azidoalanine was previously believed to be silent with regard to the cellular protein synthesis machinery.

引用

页码：10598 / 10602

页数：5

共 24 条

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